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p-Stat3 Antibody (Ser 727): sc-135649

 |  Datasheet

(Based on data analysis)

  • p-Stat3 Antibody (Ser 727) is a rabbit polyclonal IgG provided at 100 µg/ml
  • recommended for detection of Ser 727 phosphorylated Stat3 of mouse, rat and human origin by WB, IP, IF and IHC(P)
  • See 1 citations for p-Stat3 Antibody (Ser 727)
 

See additional Stat Antibodies including Stat, Stat1, Stat5A/B, Stat2, Stat3, Stat4, Stat5 and Stat6.

Also see Stat3 Inhibitors for functional analysis of cellular responses to Stat3.


Ordering InformationProduct CitationsGene Info
Recommended Support Products:
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WB   IP   IF   IHC(P)   siRNA  
Set Currency

 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
p-Stat3 Antibody (Ser 727) sc-135649 100 µg/ml $279
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
Stat3 siRNA (h) sc-29493 10 µM $258
Stat3 siRNA (m) sc-29494 10 µM $258
Stat3 (h)-PR sc-29493-PR 10 µM, 20 µl $23
Stat3 (m)-PR sc-29494-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
Stat3 shRNA Plasmid (h) sc-29493-SH 20 µg $520
Stat3 shRNA Plasmid (m) sc-29494-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
Stat3 shRNA (h) Lentiviral Particles sc-29493-V 200 µl $625
Stat3 shRNA (m) Lentiviral Particles sc-29494-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
SK-MEL-28 + IFN-γ Cell Lysate sc-2291 500 µg/200 µl $104
K-562 Whole Cell Lysate sc-2203 500 µg/200 µl $104
HeLa + IFN-γ Cell Lysate sc-2222 500 µg/200 µl $104
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
A-431 + EGF Whole Cell Lysate sc-2202 500 µg/200 µl $104
SK-MEL-28 + IFN-α Cell Lysate sc-2290 500 µg/200 µl $104
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human STAT3 6774 17q21.2 P40763
 102582
Mouse Stat3 20848 11 D P42227
 N/A
 

Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-a and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3∫ appears to be activated by both while Stat3å is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.

p-Stat3 Antibody (Ser 727) Product Citations
See how others have used p-Stat3 Antibody (Ser 727) and or p-Stat3 Antibody (Ser 727) conjugates.


1 total citations
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p-Stat3 Antibody (Ser 727) Data
Click on image to enlarge
sc-135649
p-Stat3 (Ser 727): sc-135649. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear localization.
sc-135649
Western blot analysis of phosphorylated Stat3 expression in HeLa cell extracts (A,B,C). Blots were probed with p-Stat3 (Ser 727): sc-135649 (A) and p-Stat3 (Ser 727): sc-135649 preincubated with its cognate non-phosphorylated peptide (B) and p-Stat3 (Ser 727): sc-135649 preincubated with its cognate phosphorylated peptide (C).
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