apoB Antibody (C1.4): sc-13538

apoB Antibody (C1.4) is a mouse monoclonal IgG1 kappa light chain antibody that detects the apoB protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-apoB antibody (C1.4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. apoB protein plays a crucial role in lipid metabolism, serving as a primary structural component of lipoproteins that transport cholesterol and triglycerides in the bloodstream. This function is vital for maintaining lipid homeostasis and preventing cardiovascular diseases, as imbalances in lipoprotein levels can lead to atherosclerosis and other metabolic disorders. Notably, two isoforms of apoB circulate in the plasma: apoB-100, synthesized in the liver, and apoB-48, produced in the small intestine through a unique post-transcriptional editing process. The ability of the body to produce these distinct forms of apoB from a single gene highlights the importance of RNA editing in regulating lipid metabolism and underscores the relevance of apoB monoclonal antibody (C1.4) in research focused on cardiovascular health and metabolic diseases.

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apoB Antibody (C1.4) References:

1. An AU-rich sequence element (UUUN[A/U]U) downstream of the edited C in apolipoprotein B mRNA is a high-affinity binding site for Apobec-1: binding of Apobec-1 to this motif in the 3' untranslated region of c-myc increases mRNA stability.  |  Anant, S. and Davidson, NO. 2000. Mol Cell Biol. 20: 1982-92. PMID: 10688645
2. Induction of cytidine to uridine editing on cytoplasmic apolipoprotein B mRNA by overexpressing APOBEC-1.  |  Yang, Y., et al. 2000. J Biol Chem. 275: 22663-9. PMID: 10833526
3. ApoB mRNA editing is mediated by a coordinated modulation of multiple apoB mRNA editing enzyme components.  |  Chen, Z., et al. 2007. Am J Physiol Gastrointest Liver Physiol. 292: G53-65. PMID: 16920700
4. Apolipoprotein B (APOB) gene polymorphism in patients with gallbladder disease.  |  Kurzawski, M., et al. 2007. Arch Med Res. 38: 360-3. PMID: 17350490
5. Recent progress in understanding apolipoprotein B.  |  Young, SG. 1990. Circulation. 82: 1574-94. PMID: 1977530
6. Simultaneous quantification by double rocket immunoelectrophoresis of apolipoproteins A-I and B in blood spotted on filter paper.  |  Micic, S., et al. 1988. Clin Chem. 34: 2452-5. PMID: 3143508
7. Human liver apolipoprotein B-100 cDNA: complete nucleic acid and derived amino acid sequence.  |  Law, SW., et al. 1986. Proc Natl Acad Sci U S A. 83: 8142-6. PMID: 3464946
8. Novel APOB mutation in familial hypobetalipoproteinemia.  |  Domenech, M., et al. 2022. J Clin Lipidol. 16: 28-32. PMID: 34852964
9. Two new monoclonal antibody-based enzyme-linked assays of apolipoprotein B.  |  Young, SG., et al. 1986. Clin Chem. 32: 1484-90. PMID: 3524903
10. Biosynthesis and Metabolism of ApoB-Containing Lipoproteins.  |  Borén, J., et al. 2024. Annu Rev Nutr. 44: 179-204. PMID: 38635875
11. Discordance among apoB, non-high-density lipoprotein cholesterol, and triglycerides: implications for cardiovascular prevention.  |  Sniderman, AD., et al. 2024. Eur Heart J. 45: 2410-2418. PMID: 38700053
12. Human apolipoprotein B: identification of cDNA clones and characterization of mRNA.  |  Mehrabian, M., et al. 1985. Nucleic Acids Res. 13: 6937-53. PMID: 3903660

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-apoB Antibody (C1.4) has 25 citations in a variety of scientific publications.

Primary antibodies like Anti-apoB Antibody (C1.4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

apoB Antibody (C1.4) Specifications

Alternate Product Name: Apolipoprotein B

Product Clone Name: C1.4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 97-526

Epitope Target: apoB

Epitope Species: human

Product Reactivity/Detection: apoB-100

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 25 citations in various scientific publications

Available Conjugates/Forms for apoB Antibody (C1.4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred apoB antibody is apoB Antibody (A-6): sc-393636.