epitope corresponding to amino acids 571-610 mapping near the C-terminus of ICK of mouse origin
recommended for detection of ICK of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
ICK Background Information Intestinal cell kinase (ICK), also designated MRK, LCK2, KIAA0936 and MGC46090, is a nuclear Cdc2-related kinase. ICK shares nearly identical N-terminal catalytic domains with male germ cell-associated kinase (MAK), however the C-terminal noncatalytic domain of intestinal cell kinase diverges from that of MAK. The catalytic domain of ICK is also related to mitogen-activated protein kinases (MAPKs) and harbors a corresponding TDY motif, the dual phosphorylation of which activates ICK. The phosphorylation of Tyr 159 in the TDY motif requires ICK autokinase activity, but confers only basal kinase activity; full activation of the protein requires additional phosphorylation of Thr 157 in the TDY motif.
