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home » Signaling Intermediates » Int-6 Antibody / Int-6 Antibodies » Int-6 (A-11): sc-133251

Int-6 (A-11) Antibody: sc-133251

  |  Datasheet
  • mouse monoclonal IgG1, 200µg/ml
  • raised against amino acids 246-445 mapping at the C-terminus of Int-6 of human origin
  • recommended for detection of Int-6 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine and porcine
 
Additional Int-6 Antibodies ...
 
Ordering Information
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   siRNA  
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human EIF3E 3646 8q23.1 P60228
 602210
Mouse Eif3e 16341 15 B3.2 P60229
 N/A
 
Set Currency

 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
Int-6 (A-11) sc-133251 200 µg/ml $279
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Int-6 siRNA (h) sc-40561 10 µM $258
Int-6 siRNA (m) sc-40562 10 µM $258
Int-6 (h)-PR sc-40561-PR 10 µM $23
Int-6 (m)-PR sc-40562-PR 10 µM $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Int-6 shRNA Plasmid (h) sc-40561-SH 20 µg $520
Int-6 shRNA Plasmid (m) sc-40562-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Int-6 shRNA (h) Lentiviral Particles sc-40561-V 200 µl $625
Int-6 shRNA (m) Lentiviral Particles sc-40562-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Jurkat Whole Cell Lysate sc-2204 500 µg/200 µl $104
NIH/3T3 Whole Cell Lysate sc-2210 500 µg/200 µl $104
KNRK Whole Cell Lysate sc-2214 500 µg/200 µl $104
Int-6 (h2): 293T Lysate sc-176131 100µg/200µl $205

Int-6 Background Information
Int-6, also designated eIF3e, eIF3-p46, eIF3-p48 and eukaryotic translation initiation factor 3, subunit 6, regulates translation and protein degradation through binding with three complexes: the eukaryotic translation initiation factor 3 (eIF3), the proteasome regulatory lid and the constitutive photomorphogenesis 9 signalosome. eIF3 is a complex that mediates assembly of 40S ribosomal subunits on mRNA bearing either a 5'-cap or an internal ribosome entry site (IRES). The Int-6 gene is a site of mouse mammary tumour virus (MMTV) integration in murine tumors. Reducing Int-6 expression by RNA interference in HeLa cells alters mitosis progression through defects in spindle formation, chromosome segregation and cytokinesis. These abberations appear to correlate with an inhibition of cyclin B-Cdk1 kinase activity, due to a protracted inhibitory phosphorylated state of Cdk1.
Int-6 (A-11)
Click on image to enlarge
sc-133251
Int-6 (A-11): sc-133251. Western blot analysis of Int-6 expression in non-transfected 293T: sc-117752 (A), mouse Int-6 transfected 293T: sc-121078 (B), LADMAC (C), NIH/3T3 (D) and KNRK (E) whole cell lysates.
sc-133251
Int-6 (A-11): sc-133251. Western blot analysis of Int-6 expression in non-transfected 293T: sc-117752 (A), human Int-6 transfected 293T: sc-176131 (B) and Jurkat (C) whole cell lysates.
sc-133251
Int-6 (A-11): sc-133251. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
sc-133251
Int-6 (A-11): sc-133251. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
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