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Raf-1 Antibody (C-12): sc-133

 |  Datasheet

(Based on data analysis)

  • Raf-1 Antibody (C-12) is a rabbit polyclonal IgG provided at 200 µg/ml; also available as goat IgG provided at 200 µg/ml, sc-133-G
  • epitope mapping at the C-terminus of Raf-1 of human origin
  • recommended for detection of Raf-1 p74 of mouse, rat and human origin by WB, IP, IF, KA and ELISA; also reactive with additional species, including equine, canine and bovine
  • blocking peptide, sc-133 P
  • HRP conjugate, sc-133 HRP, 200 µg/ml
  • agarose conjugate for IP studies, sc-133 AC, 500 µg/0.25 ml agarose
  • fluorescein (sc-133 FITC) and rhodamine (sc-133 TRITC) conjugates for immuno-fluorescence, 200 µg/ml
  • Alexa Fluor® 405 (sc-133 AF405), Alexa Fluor® 488 (sc-133 AF488) and Alexa Fluor® 647 (sc-133 AF647) conjugates for immunofluorescence; 100 µg/2 ml
  • See 194 citations for Raf-1 Antibody (C-12)
 

See additional Raf Antibodies including Raf, A-Raf, Raf-1 and Raf-B.

Also see chemical Raf-1 Inhibitors, chemical Raf-1 Activators or chemical Raf-1 Substrates for functional analysis of cellular responses to Raf-1.


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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
Raf-1 Antibody (C-12) sc-133 200 µg/ml $279
Raf-1 Antibody (C-12) AC sc-133 AC 500 µg/ml, 25% ag $366
Raf-1 Antibody (C-12) Alexa Fluor® 405 sc-133 AF405 100 µg/2 ml $314
Raf-1 Antibody (C-12) Alexa Fluor® 488 sc-133 AF488 100 µg/2 ml $314
Raf-1 Antibody (C-12) Alexa Fluor® 647 sc-133 AF647 100 µg/2 ml $314
Raf-1 Antibody (C-12) FITC sc-133 FITC 200 µg/ml $282
Raf-1 Antibody (C-12) HRP sc-133 HRP 200 µg/ml $279
Raf-1 (C-12) P sc-133 P
(peptide)
100 µg/0.5 ml $61
Raf-1 Antibody (C-12) TRITC sc-133 TRITC 200 µg/ml $282
Raf-1 Antibody (C-12)-G sc-133-G 200 µg/ml $279
 CRISPR/Cas9 KO and HDR Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Raf-1 CRISPR/Cas9 KO Plasmid (h) sc-400202 20 µg $345
Raf-1 HDR Plasmid (h) sc-400202-HDR 20 µg $385
Raf-1 CRISPR/Cas9 KO Plasmid (m) sc-430995 20 µg $345
Raf-1 HDR Plasmid (m) sc-430995-HDR 20 µg $385
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Raf-1 siRNA (h) sc-29462 10 µM $258
Raf-1 siRNA (m) sc-29463 10 µM $258
Raf-1 (h)-PR sc-29462-PR 10 µM, 20 µl $23
Raf-1 (m)-PR sc-29463-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Raf-1 shRNA Plasmid (h) sc-29462-SH 20 µg $520
Raf-1 shRNA Plasmid (m) sc-29463-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Raf-1 shRNA (h) Lentiviral Particles sc-29462-V 200 µl $625
Raf-1 shRNA (m) Lentiviral Particles sc-29463-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
HeLa+UV irradiated Cell Lysate sc-2221 500 µg/200 µl $104
KNRK Whole Cell Lysate sc-2214 500 µg/200 µl $104
NIH/3T3 Whole Cell Lysate sc-2210 500 µg/200 µl $104
3611-RF Whole Cell Lysate sc-2215 500 µg/200 µl $104
HeLa + PMA/PE Whole Cell Lysate sc-24808 50 µg/50 µl $104
Raf-1 (m): 293T Lysate sc-122942 100 µg/200 µl $205
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human RAF1 5894 3p25.1 NM_002880 P04049
611554
Mouse Raf1 110157 6 E3 Q99N57
N/A
 


Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk)and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-1 is activated in response to activation of a variety of tyrosine kinase receptors as well as in response to pp60v-Src expression. There is accumulating evidence that Ras p21 may play a role in activation of Raf-1 and may play the role of the messenger from membrane tyrosine kinases to Raf-1.

Raf-1 Antibody (C-12) Product Citations
See how others have used Raf-1 Antibody (C-12) and or Raf-1 Antibody (C-12) conjugates.


194 total citations
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Raf-1 Antibody (C-12) Data
Click on image to enlarge
Western blot analysis of Raf-1 expression in KNRK (A), NIH/3T3 (B), 3611-RF (C,E) and HeLa (D) whole cell lysates. Antibodies tested include Raf-1 (C-12): sc-133 (A-D) and Raf-1 (C-20): sc-227 (E).
Raf-1 (C-12): sc-133. Western blot analysis of Raf-1 expression in UV-irradiated (A) and untreated (B) HeLa whole cell lysates.
Raf-1 (C-12): sc-133. Western blot analysis of Raf-1 expression in non-transfected: sc-117752 (A) and mouse Raf-1 transfected: sc-122942 (B) 293T whole cell lysates.
Raf-1 (C-12)-G: sc-133-G. Western blot analysis of Raf-1 expression in non-transfected: sc-117752 (A) and mouse Raf-1 transfected: sc-122942 (B) 293T whole cell lysates.
Raf-1 (C-12): sc-133. Western blot analysis of Raf-1 expression in non-transfected: sc-117752 (A) and human Raf-1 transfected: sc-113164 (B) 293T whole cell lysates.
Raf-1 (C-12)-G: sc-133-G. Western blot analysis of Raf-1 expression in non-transfected: sc-117752 (A) and human Raf-1 transfected: sc-113164 (B) 293T whole cell lysates.
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