Chfr Antibody (N-15): sc-13287

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The forkhead-associated (FHA) domain was initially identified in transcription factors that have forkhead DNA-binding domains and in protein kinases, but many cell-cycle checkpoint proteins, including chfr (checkpoint with forkhead and ring finger domains) contain FHA domains. Chfr defines a checkpoint that delays entry into metaphase in response to mitotic stress. Normal primary cells and tumor cell lines that express wild-type chfr exhibit delayed entry into metaphase when centrosome separation is inhibited by mitotic stress. Additionally, chfr seems to be required for delaying prophase in human cells. The sequence of chfr is similar to that of the fission yeast DMA1, which is involved in a later mitotic checkpoint that delays a cell's exit from mitosis in response to spindle damage.