 |
- mouse monoclonal IgG2b; 200 µg/ml
- raised against amino acids 1-300 of AIF of human origin
- recommended for detection of AIF of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA
- agarose conjugate for IP studies, sc-13116 AC, 500 µg/0.25 ml agarose
- HRP conjugate, sc-13116 HRP, 200 µg/ml
- fluorescein (sc-13116 FITC) and phycoerythrin (sc-13116 PE) conjugates for intracellular FCM, 100 tests
|
|
| |
Ordering InformationProduct Citations
Recommended Support Products:
(click button of application of choice)
AIF Background Information
A key event in the apoptotic process is the opening of the mitochondrial permeability transition pore, an event that is regulated by Bcl-2 family proteins, resulting in the release of several proteins from the mitochondrial intermembrane space. Several of these proteins participate in apoptosis, including cytochrome c, procaspases 2, 3, and 9, and AIF (apoptosis-inducing factor). AIF has been shown to cause DNA fragmentation and chromatin condensation and to induce the release of cytochrome c and caspase-9 from mitochondria. Bcl-2 overexpression has been shown to prevent the release of AIF from mitochondria, but not to block its apoptogenic activity.
| AIF (E-1) Product Citations |
See how others have used AIF (E-1): sc-13116 antibody and or AIF (E-1) antibody conjugates.
48 total citations Loading citations.
|
| |
|
|
|
AIF (E-1)
Click on image to enlarge
|
|
AIF (E-1): sc-13116. Western blot analysis of AIF expression in Jurkat whole cell lysate.
AIF (E-1) PE: sc-13116 PE. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG 2b: sc-2868. AIF (E-1): sc-13116. Western blot analysis of AIF expression in non-transfected 293T: sc-117752 (A), mouse AIF transfected 293T: sc-118296 (B) and CTLL-2 (C) whole cell lysates. AIF (E-1): sc-13116. Western blot analysis of AIF expression in non-transfected 293T: sc-117752 (A), human AIF transfected 293T: sc-111943 (B) and HeLa (C) whole cell lysates. AIF (E-1): sc-13116. Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic staining of Leydig cells and cells in seminiferous ducts.
|
|
Download
|
|
