SAP 62 Antibody (4G8): sc-130563

SAP 62 Antibody (4G8) is a mouse monoclonal IgG1 antibody that detects SAP 62 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). SAP 62, also known as SF3A2, PRP11, PRPF11, or SF3a66, is a 464 amino acid protein characterized by the presence of one matrin-type zinc finger and is a crucial component of the SF3A splicing factor complex. This complex is essential for proper splicing of pre-mRNA, as SAP 62 facilitates the binding of U2 small nuclear ribonucleoprotein (snRNP) to the branchpoint sequence in pre-mRNA, thereby converting the 15S U2 snRNP into the active 17S form that directly participates in splicing events. SAP 62′s interaction with subunit 1 (SAP 114) through its N-terminus, along with binding to the 15S U2 snRNP via its zinc finger domain, underscores SAP 62′s pivotal role in RNA processing. Additionally, SAP 62 functions independently as a microtubule-binding protein, which may have implications for cellular structure and transport mechanisms. Anti-SAP 62 antibody (4G8) versatility in various applications makes this reagent an invaluable tool for researchers studying RNA splicing and related cellular processes.

The preferred SAP 62 antibody is SAP 62 Antibody (A-3): sc-390444.