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HSF2 Antibody (H-300): sc-13056

 |  Datasheet

(Based on data analysis)

  • HSF2 Antibody (H-300) is a rabbit polyclonal IgG provided at 200 µg/ml
  • epitope corresponding to amino acids 237-536 (with a deletion of amino acids 157-174) of HSF2 of human origin
  • recommended for detection of HSF2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
  • TransCruz reagent for Gel Supershift and ChIP applications, sc-13056 X, 200 µg/0.1 ml
  • See 4 citations for HSF2 Antibody (H-300)
 
Full-length HSF2 protein sequence with immunogen highlighted:
MKQSSNVPAFLSKLWTLVEETHTNEFITWSQNGQSFLVLDEQRFAKEILPKYFKHNNMASFVRQLNMYGFRKVVHIDSGIVKQERDGPVEFQHPYFKQGQDDLLENIKRKVSSSKPEENKIRQEDLTKIISSAQKVQIKQETIESRLSELKSENESLWKEVSELRAKHAQQQQVIRKIVQFIVTLVQNNQLVSLKRKRPLLLNTNGAQKKNLFQHIVKEPTDNHHHKVPHSRTEGLKPRERISDDIIIYDVTDDNADEENIPVIPETNEDVISDPSNCSQYPDIVIVEDDNEDEYAPVIQSGEQNEPARESLSSGSDGSSPLMSSAVQLNGSSSLTSEDPVTMMDSILNDNINLLGKVELLDYLDSIDCSLEDFQAMLSGRQFSIDPDLLVDLFTSSVQMNPTDYINNTKSENKGLETTKNNVVQPVSEEGRKSKSKPDKQLIQYTAFPLLAFLDGNPASSVEQASTTASSEVLSSVDKPIEVDELLDSSLDPEPTQSKLVRLEPLTEAEASEATLFYLCELAPAPLDSDMPLLDS

See additional HSF Antibodies including HSF, HSF1, HSF3, HSF2, HSF4, HSF4a, HSF4b and HSF5.

Ordering InformationProduct CitationsGene Info
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   Gel Shift   ChIP   siRNA  
Set Currency

 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
HSF2 Antibody (H-300) sc-13056 200 µg/ml $279
HSTF2 Antibody (H-300) X sc-13056 X 200 µg/0.1 ml $279
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
HSF2 siRNA (h) sc-35613 10 µM $258
HSF2 siRNA (m) sc-35614 10 µM $258
HSF2 (h)-PR sc-35613-PR 10 µM, 20 µl $23
HSTF2 (m)-PR sc-35614-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
HSF2 shRNA Plasmid (h) sc-35613-SH 20 µg $520
HSTF2 shRNA Plasmid (m) sc-35614-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
HSF2 shRNA (h) Lentiviral Particles sc-35613-V 200 µl $625
HSTF2 shRNA (m) Lentiviral Particles sc-35614-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
HeLa + heat shock Cell Lysate sc-2272 500 µg/200 µl $104
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
KNRK Whole Cell Lysate sc-2214 500 µg/200 µl $104
NIH/3T3 + heat shock Cell Lysate sc-2217 500 µg/200 µl $104
K-562 Whole Cell Lysate sc-2203 500 µg/200 µl $104
K-562 nuclear extract sc-2130 250 µg/0.05 ml $143
HSF2 (h): 293T Lysate sc-111821 100 µg/200 µl $205
HeLa nuclear extract sc-2120 250 µg/0.05 ml $143
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human HSF2 3298 6q22.31 NM_004506 Q03933
 140581
Mouse Hsf2 15500 10 B4 P38533
 N/A
 

Prokaryotic and eukaryotic cells respond to thermal and chemical stress by inducing a group of genes collectively designated heat shock genes. In eukaryotes, this gene expression is regulated primarily at the transcription level. Heat shock transcription factors (HSF, also designated HSTF) 1 and 2 are involved in this regulation. HSF1 and HSF2 are upregulated by estrogen at both the mRNA and protein level. HSF1 is normally found as a monomer whose transcriptional activity is repressed by constitutive phosphorylation. Upon activation, HSF1 forms trimers, gains DNA binding activity and is translocated to the nucleus. HSF2 activity is associated with differentiation and development; like HSF1, it binds DNA as a trimer. Both HSF1 and HSF2 are known to be induced by proteasome inhibitors of the ubiquitin pathway.

HSF2 Antibody (H-300) Product Citations
See how others have used HSF2 Antibody (H-300) and or HSF2 Antibody (H-300) conjugates.


4 total citations
Loading citations.
 
HSF2 Antibody (H-300) Data
Click on image to enlarge
sc-13056
HSF2 (H-300): sc-13056. Western blot analysis of HSF2 expression in HeLa (A), heat shock-treated HeLa (B) and K-562 (C) whole cell lysates.
sc-13056
HSF2 (H-300): sc-13056. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
sc-13056
HSF2 (H-300): sc-13056. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tissue showing cytoplasmic localization.
sc-13056
HSF2 (H-300): sc-13056. Western blot analysis of HSF2 expression in non-transfected 293T: sc-117752 (A) and human HSF2 transfected 293T: sc-111821 (B) whole cell lysates and HeLa nuclear extract (C).
sc-13056
HSF2 (H-300): sc-13056. Western blot analysis of HSF2 expression in non-transfected 293T: sc-117752 (A), human HSF2 transfected 293T: sc-111821 (B) and HeLa (C) whole cell lysates.
sc-13056
HSF2 (H-300): sc-13056. Western blot analysis of HSF2 expression in NIH/3T3 whole cell lysate.
sc-13056
HSF2 (H-300): sc-13056. Western blot analysis of HSF2 expression in non-transfected: sc-117752 (A) and mouse HSF2 transfected: sc-120906 (B) 293T whole cell lysates.
sc-13056
HSF2 (H-300): sc-13056. Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic staining of cells in seminiferous ducts and Leydig cells.
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