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- rabbit polyclonal IgG, 200 µg/ml
- epitope corresponding to amino acids 209-508 mapping at the C-terminus of SRF of human origin
- recommended for detection of SRF of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine, bovine, porcine and avian
- TransCruz reagent for Gel Supershift and ChIP applications, sc-13029 X, 200 µg/0.1 ml
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Ordering InformationProduct Citations
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| Species |
Gene Name |
Gene ID |
Chromosome Location |
Isoform (mRNA) Accession # |
Protein Accession # |
OMIM™ Number |
| Human |
SRF |
6722 |
6p21.1 |
NM_003131 |
P11831
|
600589 |
| Mouse |
Srf |
20807 |
17 C |
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Q9JM73
|
N/A |
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SRF Background Information
Serum response factor (SRF) is a transcription factor that binds the serum response element (SRE), a sequence that mediates the transient response of many cellular genes to growth stimulation. SRF-binding sites are also constitutive promotor elements in many muscle-specific promotors. At the c-Fos SRE, formation of a ternary complex containing SRF and its accessory protein p62TCF appears to be important for signal transduction. Two related Ets domain proteins, Elk-1 and SRF accessory protein-1 (SAP-1) have DNA binding properties identical to that of p62TCF. Elk-1 and SAP-1 contain two homologous regions of which the two amino terminal regions, the Ets domain (box A) and the B box, mediate ternary complex formation with SRF. The third homologous region, the C box located toward the C-terminus of the proteins, contains conserved consensus phosphorylation sites for MAP kinases.
| SRF (H-300) Product Citations |
See how others have used SRF (H-300): sc-13029 antibody and or SRF (H-300) antibody conjugates.
8 total citations Loading citations.
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SRF (H-300)
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SRF (H-300): sc-13029. Western blot analysis of SRF expression in K-562 nuclear extract.
SRF (H-300): sc-13029. Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear localization.
SRF siRNA (h): sc-36563. Western blot analysis of SRF expression in non-transfected control (A) and SRF siRNA transfected (B) K-562 cells. Blot probed with SRF (H-300): sc-13029. Actin (I-19): sc-1616 used as specificity and loading control. SRF (H-300): sc-13029. Western blot analysis of SRF expression in Jurkat whole cell lysate (A) and Jurkat whole cell lysate immunoprecipitated with SRF (A-11): sc-25290 and detected with SRF (H-300): sc-13029 (B). SRF (A-11): sc-25290. Western blot analysis of SRF isoform expression in Jurkat whole cell lysate immunoprecipitated with SRF (H-300): sc-13029 and detected with SRF (A-11): sc-25290. SRF (H-300): sc-13029. Immunoperoxidase staining of formalin fixed, paraffin-embedded human fallopian tube tissue showing nuclear staining of glandular and smooth muscle cells.
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