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p-ZAP-70 Antibody (Tyr 292): sc-12945

 |  Datasheet

(Based on data analysis)

  • p-ZAP-70 Antibody (Tyr 292) is a goat polyclonal IgG provided at 200 µg/ml; also available as rabbit IgG provided at 200 µg/ml, sc-12945-R
  • epitope corresponding to a short amino acid sequence containing phosphorylated Tyr 292 of ZAP-70 of human origin
  • recommended for detection of Tyr 292 phosphorylated ZAP-70 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, bovine and avian
  • blocking peptide, sc-12945 P
  • See 3 citations for p-ZAP-70 Antibody (Tyr 292)
 

See additional ZAP-70 Antibodies.

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 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
p-ZAP-70 Antibody (Tyr 292) sc-12945 200 µg/ml $279
p-ZAP-70 (Tyr 292) P sc-12945 P
(peptide)
 100 µg/0.5 ml $61
p-ZAP-70 Antibody (Tyr 292)-R sc-12945-R 200 µg/ml $279
Lambda Phosphatase sc-200312 100000 units in 0.25 ml $462
Lambda Phosphatase sc-200312A 20000 units in 0.05 ml $116
Lambda Phosphatase sc-200312 100000 units in 0.25 ml $462
Lambda Phosphatase sc-200312A 20000 units in 0.05 ml $116
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
ZAP-70 siRNA (h) sc-29526 10 µM $258
ZAP-70 siRNA (m) sc-36867 10 µM $258
ZAP-70 (h)-PR sc-29526-PR 10 µM, 20 µl $23
ZAP-70 (m)-PR sc-36867-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
ZAP-70 shRNA Plasmid (h) sc-29526-SH 20 µg $520
ZAP-70 shRNA Plasmid (m) sc-36867-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
ZAP-70 shRNA (h) Lentiviral Particles sc-29526-V 200 µl $625
ZAP-70 shRNA (m) Lentiviral Particles sc-36867-V 200 µl $625
 WB Positive Control Cell Lysate (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
Jurkat Whole Cell Lysate sc-2204 500 µg/200 µl $104
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human ZAP70 7535 2q11.2 NM_001079, NM_207519 P43403
 176947
Mouse Zap70 22637 1 B P43404
 N/A
 

The activation of T lymphocytes by antigens is mediated by the T cell receptor (TCR), which is a multisubunit complex assembled from at least six different genes (1,2). The TCR subunits include the Ti a and b chains, the CD3 g , d and e chains and a z -containing homodimer or heterodimer (1,2). The protein tyrosine kinase ZAP-70 binds to the phosphorylated immunoreceptor tyrosine-base activation motifs (ITAMs) of the TCR z chain through two src-homology (SH2) domains (3,4). This binding results in the phosphorylation of ZAP-70 on multiple tyrosine residues, including Tyr292 and Tyr319 (4). ZAP-70 is autophosphorylated on Tyr292, which is thought to negatively regulate ZAP-70 function in lymphocytes (5,6). Alternatively, ZAP-70 is positively regulated by phosphorylation on Tyr319, which mediates the SH2-dependent interaction between Lck and ZAP-70 (5,6).

p-ZAP-70 Antibody (Tyr 292) Product Citations
See how others have used p-ZAP-70 Antibody (Tyr 292) and or p-ZAP-70 Antibody (Tyr 292) conjugates.


3 total citations
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p-ZAP-70 Antibody (Tyr 292) Data
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sc-12945
p-ZAP-70 (Tyr 292)-R: sc-12945-R. Western blot analysis of ZAP-70 phosphorylation in untreated (A), pervanadate treated (B) and pervanadate and lambda protein phosphatase treated (C) Jurkat whole cell lysates.
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