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p-Ezrin (Tyr 146)-R Antibody: sc-12941-R |
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- rabbit polyclonal IgG, 200 µg/ml
- recommended for detection of Tyr 146 phosphorylated Ezrin of mouse, rat and human origin by WB, IP, IF and ELISA
- blocking peptide, sc-12941 P
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Ezrin Background Information
The microvillar core protein Ezrin is a tyrosine-phosphorylated protein found in epidermal growth factor-stimulated A-431 carcinoma cells (3). Ezrin is phosphorylated transiently to a high level on tyrosine residues 146 and 354 (1,2). One of the sites, Tyrosine 146, lies in the amino-terminal region of homology that is common to the Protein 4.1-Talin-Ezrin protein family (1,4). This tyrosine residue and its vicinal amino acids are conserved throughout the family which includes Radixin, Moesin, and the two phosphotyrosine phosphatases, PTP H1 and PTP MEG (1). Another phosphorylation site is Tyrosine 354, which is localized within the alpha-helical domain of Ezrin (1). Cytoplasmic signaling may result in activation of Ezrin in tyrosine phosphorylation and this suggests that Ezrin has qualities that might play a role in modulation of cell shape and adhesion (5).
| p-Ezrin (Tyr 146)-R Product Citations |
See how others have used p-Ezrin (Tyr 146)-R: sc-12941-R antibody and or p-Ezrin (Tyr 146)-R antibody conjugates.
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p-Ezrin (Tyr 146)-R
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p-Ezrin (Tyr 146)-R: sc-12941-R. Western blot analysis of Ezrin phosphorylation in Ramos whole cell lysate. p-Ezrin (Tyr 146)-R: sc-12941-R. Western blot analysis of Ezrin phosphorylation in non-transfected: sc-117752 (A), untreated human Ezrin transfected: sc-170691 (B) and lambda protein phosphatase treated human Ezrin transfected: sc-170691 (C) 293T whole cell lysates. p-Ezrin (Tyr 146)-R: sc-12941-R. Western blot analysis of Ezrin hosphorylation in untreated (A), EGF treated (B) and EGF and lambda protein phosphatase treated (C) A-431 whole cell lysates. Western blot analysis of Ezrin phosphorylation in untreated (A,C) and lambda protein phosphatase treated (B,D) Ramos whole cell lysates. Antibodies tested include p-Ezrin (Tyr 146)-R: sc-12941-R (A,B) and Ezrin (H-276): sc-20773 (C,D). Western blot analysis of Ezrin phosphorylation in untreated (A,C), and lambda protein phosphatase treated (B,D) Ramos whole cell lysates. Antibodies tested include p-Ezrin (Tyr 146)-R: sc-12941-R (A,B) and Ezrin (3C12): sc-58758 (B,D). |
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