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p-eIF4E Antibody (Ser 209): sc-12885

 |  Datasheet

(Based on data analysis)

  • p-eIF4E Antibody (Ser 209) is a goat polyclonal IgG provided at 200 µg/ml; also available as rabbit IgG provided at 200 µg/ml, sc-12885-R
  • recommended for detection of eIF4E of mouse, rat and human origin phosphorylated at Ser 209 by WB, IP, IF, IHC(P) and ELISA; also reactive with additional mammalian species, including bovine
  • blocking peptide, sc-12885 P; also reactive with additional species, including equine, canine, bovine, porcine and avian
  • See 7 citations for p-eIF4E Antibody (Ser 209)
 

See additional eIF Antibodies including eIF, eIF1, eIF1/1B, eIF1AD, eIF1AX, eIF1AY, eIF2 alpha, eIF2 beta, eIF2B alpha, eIF2B beta, eIF2B delta, eIF2B epsilon, eIF2B gamma, eIF2C, eIF2D, eIF3 alpha, eIF3 beta, eIF3 delta, eIF3 epsilon, eIF3 eta, eIF3 gamma, eIF3 theta, eIF3 zeta, eIF3e, eIF3K, eIF3M, eIF4A, eIF4AI, eIF4AII, eIF4AIII, eIF4B, eIF4E, eIF4G, eIF4GII, eIF4H, eIF5, eIF5A, eIF5B, eIF6, eIF2C1, eIF2C2, eIF2C3, eIF2S3, eIF2C4, eIF2C5, EIF3S6IP, eIF4E2, eIF4E3 and eIF3 p110.

Also see eIF4E Inhibitors for functional analysis of cellular responses to eIF4E.


Ordering InformationProduct CitationsGene Info
Recommended Support Products:
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WB   IP   IF   IHC(P)   siRNA  
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 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
p-eIF4E Antibody (Ser 209) sc-12885 200 µg/ml $279
p-eIF4E (Ser 209) P sc-12885 P
(peptide)
 100 µg/0.5 ml $61
p-eIF4E Antibody (Ser 209)-R sc-12885-R 200 µg/ml $279
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
eIF4E siRNA (h) sc-35284 10 µM $258
eIF4E siRNA (m) sc-35285 10 µM $258
eIF4E (h)-PR sc-35284-PR 10 µM, 20 µl $23
eIF4E (m)-PR sc-35285-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
eIF4E shRNA Plasmid (h) sc-35284-SH 20 µg $520
eIF4E shRNA Plasmid (m) sc-35285-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
eIF4E shRNA (h) Lentiviral Particles sc-35284-V 200 µl $625
eIF4E shRNA (m) Lentiviral Particles sc-35285-V 200 µl $625
 WB Positive Control Cell Lysate (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
NIH/3T3 + serum-starved Cell Lysate sc-2257 500 µg/200 µl $104
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human EIF4E 1977 4q23 NM_001968 P06730
 133440
Mouse Eif4e 13684 3 G3 P63073
 N/A
 

Eukaryotic initiation factor 4E (eIF4E) binds to the mRNA cap structure, thereby mediating the initiation of translation. eIF4E interacts with eIF4G, which serves as a scaffold protein for the assembly of eIF4E and eIF4A, to form the eIF4F complex. The activity of eIF4E is regulated by at least two mechanisms: inactivation through binding to 4E-BP1 in quiescent cells; and phosphorylation of eIF4E at Serine 209, which can be induced by mitogens, growth factors, hormones and some types of stress. Phosphorylation of eIF4E enhances the affinity of eIF4E for capped mRNA and for eIF4G, generating a more stable eIF4F complex. Mitogens stimulate phosphorylation of 4E-BP1 causing the release of eIF4E. Thus, eIF4E is a phosphoprotein whose phosphorylation state positively correlates with cell proliferation and growth. MAP kinase-interacting protein kinase-1, which has been shown to phosphorylate eIF4E at Serine 209, is considered a good candidate for the eIF4E kinase.

p-eIF4E Antibody (Ser 209) Product Citations
See how others have used p-eIF4E Antibody (Ser 209) and or p-eIF4E Antibody (Ser 209) conjugates.


7 total citations
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p-eIF4E Antibody (Ser 209) Data
Click on image to enlarge
sc-12885
Western blot detection of eIF4E phosphorylation in NIH/3T3 cells that were serum starved and then treated with serum. Blots were probed with eIF4E (P-2): sc-9976 (A) and p-eIF4E (Ser 209)-R: sc-12885-R (B,C). Antibody was preincubated with cognate non-phosphorylated (B) or phosphorylated (C) peptide.
sc-12885
Western blot analysis of phosphorylated elF4E expression in untreated (A,D), insulin-treated (B,E) and serum starved then, serum treated (C,F) NIH/3T3 whole cell lysates. Blots were probed with p-eIF4E (Ser 209)-R: sc-12885-R (A,B,C) and eIF4E (FL-217): sc-13963 preincubated with its cognate phosphorylated peptide (D,E,F).
sc-12885
p-eIF4E Antibody (Ser 209): sc-12885-R. Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of exocrine glandular cells and Islets of Langerhans.
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