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home » Signaling Intermediates » 4E-BP1 Antibody / 4E-BP1 Antibodies » p-4E-BP1 (Ser 65/Thr 70): sc-12884

p-4E-BP1 (Ser 65/Thr 70) Antibody: sc-12884

  |  Datasheet
  • goat polyclonal IgG, 200 µg/ml; also available as rabbit IgG, 200 µg/ml, sc-12884-R
  • epitope corresponding to a short amino acid sequence containing phosphorylated Ser 65 and Thr 70 of 4E-BP1 of human origin
  • recommended for detection of Ser 65 and Thr 70 phosphorylated 4E-BP1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
  • blocking peptide, sc-12884 P
 
Additional 4E-BP1 Antibodies ...
 
Ordering InformationProduct Citations
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   siRNA  
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human EIF4EBP1 1978 8p12 NM_004095 Q13541
 602223
Mouse Eif4ebp1 13685 8 A2 Q60876
 N/A
 
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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
p-4E-BP1 (Ser 65/Thr 70) sc-12884 200 µg/ml $279
p-4E-BP1 (Ser 65/Thr 70) P sc-12884 P
(peptide)
 100 µg/0.5 ml $61
p-4E-BP1 (Ser 65/Thr 70)-R sc-12884-R 200 µg/ml $279
Lambda Phosphatase sc-200312 100,000 units $462
Lambda Phosphatase sc-200312A 20,000 units $116
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
4E-BP1 siRNA (h) sc-29594 10 µM $258
4E-BP1 siRNA (m) sc-29595 10 µM $258
4E-BP1 (h)-PR sc-29594-PR 10 µM $23
4E-BP1 (m)-PR sc-29595-PR 10 µM $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
4E-BP1 shRNA Plasmid (h) sc-29594-SH 20 µg $520
4E-BP1 shRNA Plasmid (m) sc-29595-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
4E-BP1 shRNA (h) Lentiviral Particles sc-29594-V 200 µl $625
4E-BP1 shRNA (m) Lentiviral Particles sc-29595-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
4E-BP1 (m): 293T Lysate sc-118030 100µg/200µl $205
4E-BP1 (h): 293T Lysate sc-116590 100µg/200µl $205
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104

4E-BP1 Background Information
The multisubunit eukaryotic translation initiation factor (eIF) 4F recruits 40S ribosomal subunits to the 5' end of mRNA (1). The eIF4F subunit eIF4E interacts directly with the mRNA 5' cap structure (1). Assembly of the eIF4F complex is inhibited by a family of repressor polypeptides, the eIF4E-binding proteins (4E-BPs) (1–3). 4E-BP1 (also known as PHAS-1) normally binds eIF4E, inhibiting cap-dependent translation. Hyperphosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation (1). The PI3-kinase/Akt pathway and the FRAP/mTOR kinase regulate 4E-BP1 (2,3). 4E-BP1 is phosphorylated in vivo on multiple residues and phosphorylation by FRAP/mTOR on Threonine 37 and Threonine 46 of human 4E-BP1 may prime it for sub-sequent phosphorylation at sites including Serine 65 and Threonine 70 (4,5). The corresponding rat residues include Threonine 36, Threonine 45, Serine 64 and Threonine 69. In vitro, 4E-BP1 is also phosphorylated by ataxia telangiectasia (ATM) at human Serine 112 (rat Serine 111) in response to an increase in insulin levels (6).

p-4E-BP1 (Ser 65/Thr 70) Product Citations
See how others have used p-4E-BP1 (Ser 65/Thr 70): sc-12884 antibody and or p-4E-BP1 (Ser 65/Thr 70) antibody conjugates.


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p-4E-BP1 (Ser 65/Thr 70)
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sc-12884
p-4E-BP1 (Ser 65/Thr 70)-R: sc-12884-R. Western blot analysis of phosphorylated 4E-BP1 expression in untreated (A), insulin-treated (B) and serum starved + serum (C) NIH/3T3 whole cell lysates.
sc-12884
Western blot analysis of phosphorylated 4E-BP1 expression in untreated (A,D), insulin-treated (B,E) and serum starved + serum (C,F) NIH/3T3 whole cell lysates. Blots were probed with p-4E-BP1 (Ser 65/Thr 70): sc-12884 (A,B,C) and p-4E-BP1 (Ser 65/Thr 70): sc-12884 preincu-bated with its cognate phosphorylated peptide (D,E,F).
sc-12884
p-4E-BP1 (Ser 65/Thr 70): sc-12884. Western blot analysis of 4E-BP1 phosphorylation in non-transfected: sc-117752 (A) and mouse 4E-BP1 transfected: sc-118030 (B) 293T whole cell lysates.
sc-12884
p-4E-BP1 (Ser 65/Thr 70): sc-12884. Western blot analysis of 4E-BP1 phosphorylation in A-431 whole cell lysate.
sc-12884
p-4E-BP1 (Ser 65/Thr 70): sc-12884. Western blot analysis of 4E-BP1 phosphorylation in non-transfected: sc-117752 (A) and mouse 4E-BP1 transfected: sc-118030 (B) 293T whole cell lysates.
sc-12884
p-4E-BP1 (Ser 65/Thr 70): sc-12884. Western blot analysis of 4E-BP1 phosphorylation in non-transfected: sc-117752 (A), untreated human 4E-BP1 transfected: sc-116590 (B) and lambda protein phosphatase treated human 4E-BP1 transfected: sc-116590 (C) 293T whole cell lysates.
sc-12884
p-4E-BP1 (Ser 65/Thr 70)-R: sc-12884-R. Western blot analysis of 4E-BP1 phosphorylation in 293T (A) and HeLa (B) whole cell lysates.
sc-12884
p-4E-BP1 (Ser 65/Thr 70)-R: sc-12884-R. Western blot analysis of 4E-BP1 phosphorylation in non-transfected: sc-117752 (A), untreated human 4E-BP1 transfected: sc-116590 (B) and lambda protein phosphatase treated human 4E-BP1 transfected: sc-116590 (C) 293T whole cell lysates.
sc-12884
p-4E-BP1 (Ser 65/Thr 70)-R: sc-12884-R. Western blot analysis of 4E-BP1 expression in untreated (A) and lambda protein phosphatase treated (B) A-431 whole cell lysates.
sc-12884
Western blot analysis of 4E-BP1 phosphorylation in non-transfected: sc-117752 (A, D), untreated human 4E-BP1 transfected: sc-116590 (B, E) and lambda protein phosphatase treated human 4E-BP1 transfected: sc-116590 (C, F) 293T whole cell lysates. Antibodies tested include p-4E-BP1 (Ser 65/Thr 70): sc-12884 (A, B, C) and 4E-BP1 (R-113): sc-6936 (D, E, F).
sc-12884
Western blot analysis of 4E-BP1 phosphorylation in untreated (A), calyculin A treated (B) and calyculin A and lambda protein phosphatase (sc-200312A) treated (C) Jurkat whole cell lysates. Antibody tested is p-4E-BP1 (Ser 65/Thr 70)-R: sc-12884-R (A,B,C).
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