myogenin Antibody (F5D): sc-12732

myogenin Antibody (F5D) is a mouse monoclonal IgG1 kappa light chain antibody targeting an epitope within amino acids 138-158 of rat myogenin, with reactivity in mouse, rat, and human species. myogenin monoclonal antibody (F5D) is suitable for applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry on paraffin-embedded sections (IHCP). Myogenin, also known as MYOG, is a member of the myogenic regulatory factor (MRF) family, which includes MyoD, Myf-5, and Myf-6 (MRF4 or herculin), and plays a critical role in the differentiation of skeletal muscle cells. As a basic helix-loop-helix (bHLH) transcription factor, myogenin binds to E-box sequences (CANNTG) in the promoter regions of muscle-specific genes by forming heterodimers with E proteins such as E2A and HEB. Myogenin is expressed during the differentiation phase of myogenesis, ensuring the activation of genes required for terminal muscle cell differentiation. This function is essential for the proper formation, maintenance, and repair of skeletal muscle tissue. Dysregulation of myogenin can affect muscle development and regeneration, underscoring myogenin′s importance in muscle biology. myogenin monoclonal antibody (F5D) is a valuable tool for investigating the molecular mechanisms governing myogenesis and skeletal muscle development.

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myogenin Antibody (F5D) References:

1. FHL3 binds MyoD and negatively regulates myotube formation.  |  Cottle, DL., et al. 2007. J Cell Sci. 120: 1423-35. PMID: 17389685
2. Rhabdomyosarcoma subtyping by immunohistochemical assessment of myogenin: tissue array study and review of the literature.  |  Morgenstern, DA., et al. 2008. Pathol Oncol Res. 14: 233-8. PMID: 18493875
3. Herculin, a fourth member of the MyoD family of myogenic regulatory genes.  |  Miner, JH. and Wold, B. 1990. Proc Natl Acad Sci U S A. 87: 1089-93. PMID: 2300571
4. Myf-6, a new member of the human gene family of myogenic determination factors: evidence for a gene cluster on chromosome 12.  |  Braun, T., et al. 1990. EMBO J. 9: 821-31. PMID: 2311584
5. Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD.  |  Wright, WE., et al. 1989. Cell. 56: 607-17. PMID: 2537150
6. Identification of MRF4: a new member of the muscle regulatory factor gene family.  |  Rhodes, SJ. and Konieczny, SF. 1989. Genes Dev. 3: 2050-61. PMID: 2560751
7. A novel human muscle factor related to but distinct from MyoD1 induces myogenic conversion in 10T1/2 fibroblasts.  |  Braun, T., et al. 1989. EMBO J. 8: 701-9. PMID: 2721498
8. Dissecting myogenin-mediated retinoid X receptor signaling in myogenic differentiation.  |  Khilji, S., et al. 2020. Commun Biol. 3: 315. PMID: 32555436
9. Myogenin controls via AKAP6 non-centrosomal microtubule-organizing center formation at the nuclear envelope.  |  Becker, R., et al. 2021. Elife. 10: PMID: 34605406
10. HLH forced dimers: tethering MyoD to E47 generates a dominant positive myogenic factor insulated from negative regulation by Id.  |  Neuhold, LA. and Wold, B. 1993. Cell. 74: 1033-42. PMID: 7691411
11. A cellular factor stimulates the DNA-binding activity of MyoD and E47.  |  Thayer, MJ. and Weintraub, H. 1993. Proc Natl Acad Sci U S A. 90: 6483-7. PMID: 8393567
12. Use of a conditional MyoD transcription factor in studies of MyoD trans-activation and muscle determination.  |  Hollenberg, SM., et al. 1993. Proc Natl Acad Sci U S A. 90: 8028-32. PMID: 8396258

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-myogenin Antibody (F5D) has 445 citations in a variety of scientific publications.

Primary antibodies like Anti-myogenin Antibody (F5D) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

myogenin Antibody (F5D) Specifications

Alternate Product Name: myogenin

Product Clone Name: F5D

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 138-158

Epitope Target: myogenin

Epitope Species: rat

Product Reactivity/Detection: myogenin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 445 citations in various scientific publications

Available Conjugates/Forms for myogenin Antibody (F5D): AC: Agarose, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, X: TransCruz Reagent