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CD47 Antibody (B6H12): sc-12730

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  • CD47 Antibody (B6H12) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
  • raised from intact CD47 purified from placenta
  • recommended for detection of the immunoglobulin domain of CD47 of mouse, rat, human and primate origin by WB, IP, IF, IHC(P) and FCM
  • agarose (sc-12730 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-12730 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-12730 PE), fluorescein (sc-12730 FITC), Alexa Fluor® 488 (sc-12730 AF488) or Alexa Fluor® 647 (sc-12730 AF647), 200 µg/ml, for IF, IHC(P) and FCM
  • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP): sc-516102, our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
  • See 4 citations for CD47 Antibody (B6H12)
 

See additional CD47 Antibodies.

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WB   IP   IF   IHC(P)   FCM   siRNA  
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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
CD47 Antibody (B6H12) sc-12730 200 µg/ml $279
CD47 Antibody (B6H12) Alexa Fluor® 488 sc-12730 AF488 200 µg/ml $314
CD47 Antibody (B6H12) Alexa Fluor® 647 sc-12730 AF647 100 µg/2 ml $314
CD47 Antibody (B6H12) FITC sc-12730 FITC 200 µg/ml $292
CD47 Antibody (B6H12) HRP sc-12730 HRP 200 µg/ml $279
CD47 Antibody (B6H12) PE sc-12730 PE 200 µg/ml $303
 CRISPR KO, HDR and Nickase Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
CD47 CRISPR/Cas9 KO Plasmid (h) sc-400508 20 µg $345
CD47 HDR Plasmid (h) sc-400508-HDR 20 µg $385
CD47 Double Nickase Plasmid (h) sc-400508-NIC 20 µg $355
CD47 Double Nickase Plasmid (h2) sc-400508-NIC-2 20 µg $355
CD47 CRISPR/Cas9 KO Plasmid (m) sc-421180 20 µg $345
CD47 HDR Plasmid (m) sc-421180-HDR 20 µg $385
CD47 Double Nickase Plasmid (m) sc-421180-NIC 20 µg $355
CD47 Double Nickase Plasmid (m2) sc-421180-NIC-2 20 µg $355
 CRISPR Activation Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
CD47 CRISPR Activation Plasmid (h) sc-400508-ACT 20 µg $345
CD47 CRISPR Activation Plasmid (h2) sc-400508-ACT-2 20 µg $345
CD47 Lentiviral Activation Particles (h) sc-400508-LAC 200 µl $395
CD47 Lentiviral Activation Particles (h2) sc-400508-LAC-2 200 µl $395
CD47 CRISPR Activation Plasmid (m) sc-421180-ACT 20 µg $345
CD47 CRISPR Activation Plasmid (m2) sc-421180-ACT-2 20 µg $345
CD47 Lentiviral Activation Particles (m) sc-421180-LAC 200 µl $395
CD47 Lentiviral Activation Particles (m2) sc-421180-LAC-2 200 µl $395
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
CD47 siRNA (h) sc-35006 10 µM $258
CD47 siRNA (m) sc-35007 10 µM $258
CD47 (h)-PR sc-35006-PR 10 µM, 20 µl $23
CD47 (m)-PR sc-35007-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
CD47 shRNA Plasmid (h) sc-35006-SH 20 µg $520
CD47 shRNA Plasmid (m) sc-35007-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
CD47 shRNA (h) Lentiviral Particles sc-35006-V 200 µl $625
CD47 shRNA (m) Lentiviral Particles sc-35007-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
ECV304 Cell Lysate sc-2269 500 µg/200 µl $104
HISM Cell Lysate sc-2229 500 µg/200 µl $104
Jurkat Whole Cell Lysate sc-2204 500 µg/200 µl $104
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human CD47 961 3q13.13 NM_001777, NM_198793 Q08722
601028
Mouse Cd47 16423 16 B5 NM_010581 Q61735
N/A
 


CD47 is an integral membrane protein that plays a role in the regulation of cation fluxes across cell membranes. Specifically, CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to the extracellular matrix. It is also a receptor for the C-terminal cell binding domain of thrombospondin (SIRP). CD47 is absent from Rh-null erythrocytes, but does play a role in cell adhesion in non-erythroid cells and may prevent premature elimination of erythrocytes. It may also be involved in membrane permeability changes following viral infection. CD47 is expressed on hemopoietic cells, epithelial cells, endothelial cells and fibroblasts and is strongly expressed in brain and mesenchymal cells.

CD47 Antibody (B6H12) Product Reviews

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CD47 Antibody (B6H12) Product Citations
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CD47 Antibody (B6H12) Data
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CD47 (B6H12): sc-12730: Western blot analysis of CD47 expression in non-transfected HEK 293T (A) and puromycin treated, CD47 CRISPR/Cas9 KO Plasmid (h): sc-400508 plus CD47 HDR Plasmid (h): sc-400508-HDR co-transfected HEK 293T (B) whole cell lysates. β-Actin (C4): sc-47778 used as specificity and loading control.
CD47 (B6H12): sc-12730. Western blot analysis of CD47 expression in human platelet extract.
CD47 (B6H12): sc-12730. Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.
CD47 (B6H12): sc-12730. Western blot analysis of CD47 expression in non-transfected: sc-110760 (A) and human CD47 transfected: sc-112975 (B) 293 whole cell lysates.
CD47 (B6H12) FITC: sc-12730 FITC. FCM analysis of Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG1: sc-2855.
CD47 (B6H12): sc-12730. Western blot analysis of CD47 expression in non-transfected: sc-110760 (A) and human CD47 transfected: sc-112975 (B) 293 whole cell lysates.
CD47 (B6H12): sc-12730. Immunoperoxidase staining of formalin fixed, paraffin-embedded human skin cancer tissue showing membrane staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
CD47 (B6H12) PE: sc-12730 PE. FCM analysis of Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG1: sc-2866.
CD47 (B6H12): sc-12730. Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.
CD47 (B6H12) PE: sc-12730 PE. Direct immunofluorescence staining of formalin-fixed HeLa cells showing membrane localization.
Lamin A/C (636) PE: sc-7292 PE and CD47 (B6H12) FITC sc-12730 FITC. Direct immunofluorescence staining of formalin-fixed HeLa cells showing nuclear envelope (red) and membrane (green) localization.
C23 (MS-3) Alexa Fluor® 488: sc-8031 AF488 and CD47 (B6H12) PE: sc-12730 PE. Direct immunofluorescence staining of formalin-fixed HeLa cells showing nucleolar and nuclear (green) and membrane (red) localization.
CD47 (B6H12): sc-12730. Western blot analysis of CD47 expression in HEK293T (A) and Jurkat (B) whole cell lysates.
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