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- goat polyclonal IgG, 200 µg/ml
- epitope mapping near the N-terminus of mature Smac of human origin
- recommended for detection of precursor and mature Smac of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine and porcine
- blocking peptide, sc-12683 P
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Smac Background Information
The activation of caspases is a key regulatory step in apoptosis. Once cytochrome c is released from the mitochondria into the cytosol, it binds Apaf-1 to form an oligomeric cytochrome c/Apaf-1 complex, which induces caspase activation. Inhibitors of Apoptosis Proteins (IAPs), are a family of proteins that regulate the cytochrome c/Apaf-1 caspase activating pathway. Like cytochrome c, Smac (for second mitochondria-derived activator of caspase, also designated DIABLO in mouse for direct IAP binding protein with low PI) promotes caspase activation in the cytochrome c/Apaf-1/caspase-9 pathway by binding IAPs and preventing them from inhibiting caspases. In healthy cells, Smac is a mitochondrial protein, but when cells undergo apoptosis, Smac is released into the cytosol.
| Smac (V-17) Product Citations |
See how others have used Smac (V-17): sc-12683 antibody and or Smac (V-17) antibody conjugates.
9 total citations Loading citations.
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Smac (V-17)
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Smac (V-17): sc-12683. Western blot analysis of Smac expression in HeLa (A), Caki-1 (B) and DU 145 (C) whole cell lysates.
Smac (V-17): sc-12683. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining.
Smac siRNA (h): sc-36505. Immunofluorescence staining of methanol-fixed, control HeLa (A) and Smac siRNA silenced HeLa (B) cells showing diminished cytoplasmic staining in the siRNA silenced cells. Cells probed with Smac (V-17): sc-12683. |
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