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p-Adducin Antibody (Ser 662): sc-12614

 |  Datasheet

(Based on data analysis)

  • p-Adducin Antibody (Ser 662) is a goat polyclonal IgG provided at 200 µg/ml; also available as rabbit IgG provided at 200 µg/ml, sc-12614-R
  • recommended for detection of Adducin γ phosphorylated at Ser 662, Adducin α phosphorylated at Ser 724, Adducin β phosphorylated at Ser 13 of mouse, rat and human origin by WB, IP and IF
  • blocking peptide, sc-12614 P
 

See additional Adducin Antibodies including Adducin, Adducin alpha, Adducin beta and Adducin gamma.

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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
p-Adducin Antibody (Ser 662) sc-12614 200 µg/ml $279
p-Adducin (Ser 662) P sc-12614 P
(peptide)
100 µg/0.5 ml $61
p-Adducin Antibody (Ser 662)-R sc-12614-R 200 µg/ml $279
Lambda Phosphatase sc-200312 100000 units in 0.25 ml $462
Lambda Phosphatase sc-200312A 20000 units in 0.05 ml $116
Lambda Phosphatase sc-200312 100000 units in 0.25 ml $462
Lambda Phosphatase sc-200312A 20000 units in 0.05 ml $116
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
Adducin α (m): 293T Lysate sc-118250 100 µg/200 µl $205
Adducin β (h2): 293T Lysate sc-117077 100 µg/200 µl $205
Adducin γ(m): 293T Lysate sc-118249 100 µg/200 µl $205
NIH/3T3 Whole Cell Lysate sc-2210 500 µg/200 µl $104
SK-N-MC Cell Lysate sc-2237 500 µg/200 µl $104
mouse brain extract sc-2253 500 µg/200 µl $104
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human ADD1 118 4p16.3 NM_001119, NM_014189, NM_014190, NM_176801 P35611
145500
Human ADD2 119 2p13.3 NM_001617, NM_017482, NM_017483, NM_017484, NM_017488 P35612
102681
Human ADD3 120 10q25.1 NM_001121, NM_016824, NM_019903 Q9UEY8
601568
Mouse Add1 11518 5 B2 Q9QYC0
N/A
Mouse Add2 11519 6 D1 Q9QYB8
N/A
Mouse Add3 27360 19 D2 Q9QYB5
N/A
 


Adducins are a family of cytoskeleton proteins encoded by three genes (a, b and g). Adducin is a protein associated with the inner leaflet of the plasma membrane and is one of the proteins localized at the spectrin-actin junction of the membrane skeleton. Adducins promote association of spectrin with actin and cap the fast growing end of actin filaments. Adducins contain an N-terminal core, neck and C-terminal tail domains, are substrates for protein kinase A (PKA) and C (PKC), and bind to Ca2+/calmodulin. The major phosphorylation sites common to the Adducins are Ser 726 and Ser 713 in the C-terminal MARCKS-related domains of Adducin a and Adducin b, and they are phosphorylated by PKA and PKC, respectively. In addition, PKA phosphorylates Adducin a at Ser 408, 436 and 481. Calmodulin-binding is inhibited by phosphorylation of Adducin b that, in turn, inhibits the rate of phospho-rylation of Adducin b, but not Adducin a. Rho-kinase can phosphorylate Adducin a at Thr 445 and Thr 480 downstream of Rho in vivo. The phosphorylation of Adducin by Rho-kinase plays an important role in the regulation of membrane ruffling and cell motility. In addition, phosphorylation at Ser 726 of Adducin a is required for cleavage by caspase-3 .

p-Adducin Antibody (Ser 662) Data
Click on image to enlarge
Western blot detection of Adducin phosphorylation in NIH/3T3 cells. Blots were probed with Adducin (H-16): sc-11884 (A) and p-Adducin (Ser 662)-R: sc-12614-R (B,C). The antibody was preincubated with cognate non-phosphorylated (B) or phosphorylated (C) peptide.
p-Adducin (Ser 662)-R: sc-12614-R. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoskeletal localization.
p-Adducin (Ser 662): sc-12614. Western blot analysis of Adducin β phosphorylation in SK-N-MC whole cell lysate.
p-Adducin (Ser 662): sc-12614. Western blot analysis of Adducin β prosphorylation in non-transfected 293T: sc-117752 (A) and human Adducin β transfected 293T: sc-117077 (B) whole cell lysates and mouse brain tissue extract (C).
p-Adducin (Ser 662): sc-12614. Western blot analysis of Adducin γ phosphorylation in non-transfected: sc-117752 (A) and mouse Adducin γ transfected: sc-118249 (B) 293T whole cell lysates.
p-Adducin (Ser 662): sc-12614. Western blot analysis of Adducin phosphorylation in NIH/3T3 whole cell lysate.
p-Adducin (Ser 662): sc-12614. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoskeletal localization.
p-Adducin (Ser 662): sc-12614. Western blot analysis of Adducin α phosphorylation in non-transfected: sc-117752 (A) and mouse Adducin α transfected: sc-118250 (B) 293T whole cell lysates.
Western blot analysis of Adducin α phosphorylation in non-transfected: sc-117752 (A, D), untreated mouse Adducin α transfected: sc-118250 (B, E) and lambda protein phosphatase treated mouse Adducin α transfected: sc-118250 (C, F) 293T whole cell lysates. Antibodies tested include p-Adducin (Ser 662): sc-12614 (A, B, C) and Adducin α (4D1): sc-33633 (D, E, F).
Western blot analysis of Adducin α phosphorylation in non-transfected: sc-117752 (A, D), untreated mouse Adducin α transfected: sc-118250 (B, E) and lambda protein phosphatase treated mouse Adducin α transfected: sc-118250 (C, F) 293T whole cell lysates. Antibodies tested include p-Adducin (Ser 662)-R: sc-12614-R (A, B, C) and Adducin α (4D1): sc-33633 (D, E, F).
Western blot analysis of Adducin phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) HL-60 whole cell lysates. Antibodies tested include p-Adducin (Ser 662)-R: sc-12614-R (A,B) and Adducin α (H-100): sc-25731 (C,D).
Western blot analysis of Adducin phosphorylation in untreated (A,C) and lambda protein phosphatase (sc-200312A) treated (B,D) rat brain tissue extracts. Antibodies tested include p-Adducin (Ser 662)-R: sc-12614-R (A,B) and Adducin α (H-100): sc-25731 (C,D).
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