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p-Adducin (Ser 662) Antibody: sc-12614 |
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- goat polyclonal IgG, 200 µg/ml; also available as rabbit IgG, 200 µg/ml, sc-12614-R
- recommended for detection of Adducin γ phosphorylated at Ser 662, Adducin α phosphorylated at Ser 724, Adducin β phosphorylated at Ser 13 of mouse, rat and human origin by WB, IP and IF
- blocking peptide, sc-12614 P
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Adducin Background Information
Adducins are a family of cytoskeleton proteins encoded by three genes (a, b and g). Adducin is a protein associated with the inner leaflet of the plasma membrane and is one of the proteins localized at the spectrin-actin junction of the membrane skeleton. Adducins promote association of spectrin with actin and cap the fast growing end of actin filaments. Adducins contain an N-terminal core, neck and C-terminal tail domains, are substrates for protein kinase A (PKA) and C (PKC), and bind to Ca2+/calmodulin. The major phosphorylation sites common to the Adducins are Ser 726 and Ser 713 in the C-terminal MARCKS-related domains of Adducin a and Adducin b, and they are phosphorylated by PKA and PKC, respectively. In addition, PKA phosphorylates Adducin a at Ser 408, 436 and 481. Calmodulin-binding is inhibited by phosphorylation of Adducin b that, in turn, inhibits the rate of phospho-rylation of Adducin b, but not Adducin a. Rho-kinase can phosphorylate Adducin a at Thr 445 and Thr 480 downstream of Rho in vivo. The phosphorylation of Adducin by Rho-kinase plays an important role in the regulation of membrane ruffling and cell motility. In addition, phosphorylation at Ser 726 of Adducin a is required for cleavage by caspase-3 .
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p-Adducin (Ser 662)
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Western blot detection of Adducin phosphorylation in NIH/3T3 cells. Blots were probed with Adducin (H-16): sc-11884 (A) and p-Adducin (Ser 662)-R: sc-12614-R (B,C). The antibody was preincubated with cognate non-phosphorylated (B) or phosphorylated (C) peptide. p-Adducin (Ser 662)-R: sc-12614-R. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoskeleton localization.
p-Adducin (Ser 662): sc-12614. Western blot analysis of Adducin β phosphorylation in SK-N-MC whole cell lysate.
p-Adducin (Ser 662): sc-12614. Western blot analysis of Adducin prosphorylation in non-transfected 293T: sc-117752 (A) and human Adducin transfected 293T: sc-117077 (B) whole cell lysates and mouse brain tissue extract (C). p-Adducin (Ser 662): sc-12614. Western blot analysis of Adducin γ phosphorylation in non-transfected: sc-117752 (A) and mouse Adducin γ transfected: sc-118249 (B) 293T whole cell lysates. p-Adducin (Ser 662): sc-12614. Western blot analysis of Adducin phosphorylation in NIH/3T3 whole cell lysate.
p-Adducin (Ser 662): sc-12614. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoskeletal localization.
p-Adducin (Ser 662): sc-12614. Western blot analysis of Adducin α phosphorylation in non-transfected: sc-117752 (A) and mouse Adducin α transfected: sc-118250 (B) 293T whole cell lysates. Western blot analysis of Adducin α phosphorylation in non-transfected: sc-117752 (A, D), untreated mouse Adducin α transfected: sc-118250 (B, E) and lambda protein phosphatase treated mouse Adducin α transfected: sc-118250 (C, F) 293T whole cell lysates. Antibodies tested include p-Adducin (Ser 662): sc-12614 (A, B, C) and Adducin α (4D1): sc-33633 (D, E, F). Western blot analysis of Adducin α phosphorylation in non-transfected: sc-117752 (A, D), untreated mouse Adducin α transfected: sc-118250 (B, E) and lambda protein phosphatase treated mouse Adducin α transfected: sc-118250 (C, F) 293T whole cell lysates. Antibodies tested include p-Adducin (Ser 662)-R: sc-12614-R (A, B, C) and Adducin α (4D1): sc-33633 (D, E, F). |
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