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- mouse monoclonal IgG2a; 200 µg/ml
- N-terminal epitope mapping between amino acid residues 11-25 of p53 of human origin
- recommended for detection of wt and mutant p53 of human origin under denaturing and non-denaturing conditions by WB, IP, IF, IHC(P) and FCM
- agarose conjugate for IP studies, p53 (DO-1) AC: sc-126 AC, 500 µg/0.25 ml agarose
- phycoerythrin (sc-126 PE), PerCP (sc-126 PerCP) and PerCP-Cy5.5 (sc-126 PCPC5) conjugates for intracellular FCM, 100 tests
- HRP conjugate, p53 (DO-1) HRP: sc-126 HRP, 200 µg/ml
- TransCruz reagent for Gel Supershift and ChIP applications, p53 (DO-1) X: sc-126 X, 200 µg/0.1 ml
- fluorescein (p53 (DO-1) FITC: sc-126 FITC) conjugate for immunohistochemistry, 200 µg/ml
- rhodamine (p53 (DO-1) TRITC: sc-126 TRITC) conjugate for immunofluorescence, 200 µg/ml
- Alexa Fluor® 405(sc-126 AF405), Alexa Fluor® 488 (sc-126 AF488) and Alexa Fluor® 647 (sc-126 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
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Ordering InformationProduct Citations
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| Species |
Gene Name |
Gene ID |
Chromosome Location |
Isoform (mRNA) Accession # |
Protein Accession # |
OMIM™ Number |
| Human |
TP53 |
7157 |
17p13.1 |
NM_000546 |
P04637
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260350 |
| Mouse |
Trp53 |
22059 |
11 B3 |
|
P02340
|
N/A |
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p53 Background Information
p53, a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator, MDM2. MDM2 is an E3 ubiquitin ligase that is upregulated in the presence of active p53, where it poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) of p53, amino acids 110-286, can compromise energetically-favorable association with cis elements and are implicated in several human cancers.
| p53 (DO-1) Product Citations |
See how others have used p53 (DO-1): sc-126 antibody and or p53 (DO-1) antibody conjugates.
734 total citations Loading citations.
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p53 (DO-1)
Click on image to enlarge
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p53 (DO-1): sc-126. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue(A). Note intense nuclear staining of ductal epithelia. Immunofluorescence staining of methanol-fixed A-431 cells showing nuclear localization (B).
p53 (DO-1) PE: sc-126 PE. Intracellular FCM analysis of fixed and permeabilized A431 cells. Black line histogram represents the isotype control, normal mouse IgG 2a: sc-2867. p53 siRNA (h): sc-29435. Western blot analysis of p53 expression in control non-transfected (A) and p53 siRNA transfected (B) HeLa cells. Blot probed with p53 (D0-1): sc-126. α-actinin (H-2): sc-17829 used as specificity and loading control. p53 (DO-1): sc-126. Western blot analysis of p53 expression in A-431 whole cell lysate.
p53 (DO-1): sc-126. Western blot analysis of p53 expression in non-transfected: sc-117752 (A) and human p53 transfected: sc-158802 (B) 293T whole cell lysates. p53 (DO-1) FITC: sc-126 FITC. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG 2a: sc-2856. p53 (DO-1): sc-126. Western blot analysis of p53 expression in untreated (A) and Tenovin-1 ( sc-222342) treated (B) MCF7 whole cell lysates. Note upregulation of p53 expression in lane B. p53 (DO-1): sc-126. Western blot analysis of p53 expression in untreated (A) and Actinomycin D ( sc-200906) treated (B) MOLT-4 whole cell lysates. p53 (DO-1): sc-126. Western blot analysis of p53 expression in untreated (A) and SN 38 ( sc-203697) treated (B) HCT 116 whole cell lysates. Note upregulation of p53 expression in lane B. p53 (DO-1): sc-126. Western blot analysis of p53 expression in untreated (A) and RITA ( sc-202753) treated (B) HCT 116 whole cell lysates. Note upregulation of p53 expression in lane B. |
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