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p53 Antibody (DO-1): sc-126

 |  Datasheet

(Based on data analysis)

  • p53 Antibody (DO-1) is a mouse monoclonal IgG2a provided at 200 µg/ml
  • N-terminal epitope mapping between amino acid residues 11-25 of p53 of human origin
  • recommended for detection of wt and mutant p53 of human origin under denaturing and non-denaturing conditions by WB, IP, IF, IHC(P) and FCM
  • agarose conjugate for IP studies, p53 Antibody (DO-1) AC: sc-126 AC, 500 µg/0.25 ml agarose
  • phycoerythrin (sc-126 PE), PerCP (sc-126 PerCP) and PerCP-Cy5.5 (sc-126 PCPC5) conjugates for intracellular FCM, 100 tests
  • HRP conjugate, p53 Antibody (DO-1) HRP: sc-126 HRP, 200 µg/ml
  • TransCruz reagent for Gel Supershift and ChIP applications, p53 Antibody (DO-1) X: sc-126 X, 200 µg/0.1 ml
  • fluorescein (p53 Antibody (DO-1) FITC: sc-126 FITC) conjugate for immunohistochemistry, 200 µg/ml
  • rhodamine (p53 Antibody (DO-1) TRITC: sc-126 TRITC) conjugate for immunofluorescence, 200 µg/ml
  • Alexa Fluor® 405 (sc-126 AF405), Alexa Fluor® 488 (sc-126 AF488) and Alexa Fluor® 647 (sc-126 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
  • See 1010 citations for p53 Antibody (DO-1)
 

See additional p53 Antibodies.

Also see chemical p53 Inhibitors or chemical p53 Activators for functional analysis of cellular responses to p53.


Ordering InformationProduct Citations
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   FCM   Gel Shift   ChIP   siRNA  
Set Currency

 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
p53 Antibody (DO-1) sc-126 200 µg/ml $279
p53 Antibody (DO-1) AC sc-126 AC 500 µg/ml, 25% ag $366
p53 Antibody (DO-1) Alexa Fluor® 405 sc-126 AF405 100 µg/2 ml $314
p53 Antibody (DO-1) Alexa Fluor® 488 sc-126 AF488 100 µg/2 ml $314
p53 Antibody (DO-1) Alexa Fluor® 647 sc-126 AF647 100 µg/2 ml $314
p53 Antibody (DO-1) FITC sc-126 FITC 200 µg/ml $282
p53 Antibody (DO-1) HRP sc-126 HRP 200 µg/ml $279
p53 Antibody (DO-1) PCPC5 sc-126 PCPC5 100 tests in 2 ml $310
p53 Antibody (DO-1) PE sc-126 PE 100 tests in 2 ml $303
p53 Antibody (DO-1) PerCP sc-126 PerCP 100 tests in 2 ml $310
p53 Antibody (DO-1) TRITC sc-126 TRITC 200 µg/ml $282
p53 Antibody (DO-1) X sc-126 X 200 µg/0.1 ml $279
 CRISPR/Cas9 KO and HDR Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
p53 CRISPR/Cas9 KO Plasmid (h) sc-416469 20 µg $345
p53 HDR Plasmid (h) sc-416469-HDR 20 µg $385
p53 CRISPR/Cas9 KO Plasmid (m) sc-423509 20 µg $345
p53 HDR Plasmid (m) sc-423509-HDR 20 µg $385
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
p53 siRNA (h) sc-29435 10 µM $258
p53 siRNA (h2) sc-44218 10 µM $258
p53 (h)-PR sc-29435-PR 10 µM, 20 µl $23
p53 (h2)-PR sc-44218-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
p53 shRNA Plasmid (h) sc-29435-SH 20 µg $520
p53 shRNA Plasmid (h2) sc-44218-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
p53 shRNA (h) Lentiviral Particles sc-29435-V 200 µl $625
p53 shRNA (h2) Lentiviral Particles sc-44218-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
A-431 Whole Cell Lysate sc-2201 500 µg/200 µl $104
BT-20 Cell Lysate sc-2223 500 µg/200 µl $104
A549 Cell Lysate sc-2413 500 µg/200 µl $104
MDA-MB-231 Cell Lysate sc-2232 500 µg/200 µl $104
Jurkat Whole Cell Lysate sc-2204 500 µg/200 µl $104
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
MCF7 Whole Cell Lysate sc-2206 500 µg/200 µl $104


p53, a DNA-binding, oligomerization domain- and transcription activation domain-containing tumor suppressor, upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation, and cell cycle control mechanisms. p53 localizes to the nucleus, yet can be chaperoned to the cytoplasm by the negative regulator, MDM2. MDM2 is an E3 ubiquitin ligase that is upregulated in the presence of active p53, where it poly-ubiquitinates p53 for proteasome targeting. p53 fluctuates between latent and active DNA-binding conformations and is differentially activated through posttranslational modifications, including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) of p53, amino acids 110-286, can compromise energetically-favorable association with cis elements and are implicated in several human cancers.

p53 Antibody (DO-1) Product Citations
See how others have used p53 Antibody (DO-1) and or p53 Antibody (DO-1) conjugates.


1010 total citations
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p53 Antibody (DO-1) Data
Click on image to enlarge
p53 (DO-1): sc-126. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue. Note intense nuclear staining of ductal epithelia.
p53 (DO-1): sc-126. Immunofluorescence staining of methanol-fixed A-431 cells showing nuclear localization.
p53 (DO-1) PE: sc-126 PE. Intracellular FCM analysis of fixed and permeabilized A431 cells. Black line histogram represents the isotype control, normal mouse IgG2a: sc-2867.
p53 siRNA (h): sc-29435. Western blot analysis of p53 expression in control non-transfected (A) and p53 siRNA transfected (B) HeLa cells. Blot probed with p53 (D0-1): sc-126. α-actinin (H-2): sc-17829 used as specificity and loading control.
p53 (DO-1): sc-126. Western blot analysis of p53 expression in A-431 whole cell lysate.
p53 (DO-1): sc-126. Western blot analysis of p53 expression in non-transfected: sc-117752 (A) and human p53 transfected: sc-158802 (B) 293T whole cell lysates.
p53 (DO-1) FITC: sc-126 FITC. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG2a: sc-2856.
p53 (DO-1): sc-126. Western blot analysis of p53 expression in untreated (A) and Tenovin-1 (sc-222342) treated (B) MCF7 whole cell lysates. Note upregulation of p53 expression in lane B.
p53 (DO-1): sc-126. Western blot analysis of p53 expression in untreated (A) and Actinomycin D (sc-200906) treated (B) MOLT-4 whole cell lysates.
p53 (DO-1): sc-126. Western blot analysis of p53 expression in untreated (A) and SN 38 (sc-203697) treated (B) HCT 116 whole cell lysates. Note upregulation of p53 expression in lane B.
p53 (DO-1): sc-126. Western blot analysis of p53 expression in untreated (A) and RITA (sc-202753) treated (B) HCT 116 whole cell lysates. Note upregulation of p53 expression in lane B.
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