TFIIA-α (F-15) Antibody: sc-12369
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goat polyclonal IgG, 200 µg/ml epitope mapping near the N-terminus of TFIIA-α of human origin recommended for detection of TFIIA-α (35 kDa) and p55 precursor of mouse, rat and human origin by WB, IF and ELISA; also reactive with additional species, including equine, canine, bovine and avian blocking peptide, sc-12369 P TransCruz reagent for Gel Supershift and ChIP applications, sc-12369 X, 200 µg/0.1 ml
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TFIIA-α Background Information Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors (1). Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in the formation of a multiprotein complex containing several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFIIH (1-3). Recognition of the TATA binding element by TBP, one of the first steps in transcription initiation, may be regulated by TFIIA (4,5). TFIIA consists of three subunits designated TFIIA-å, TFIIA-∫ and TFIIA-©, and it interacts with both TBP and TAF (TBP-associated factor) (6,7). It has been demonstrated that the basic region of TBP is essential for TFIIA-dependent function of TBP (8).
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