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p-PKC α Antibody (Ser 657): sc-12356

 |  Datasheet

(Based on data analysis)

  • p-PKC α Antibody (Ser 657) is a goat polyclonal IgG provided at 200 µg/ml; also available as rabbit IgG provided at 200 µg/ml, sc-12356-R
  • recommended for detection of PKC α of mouse, rat and human origin phosphorylated at Ser 657 by WB, IP, IF and ELISA
  • blocking peptide, sc-12356 P; also reactive with additional species, including equine, canine, bovine and avian
  • See 21 citations for p-PKC α Antibody (Ser 657)
 

See additional PKC Antibodies including PKC, PKC alpha, PKC beta, PKC delta, PKC epsilon, PKC eta, PKC gamma, PKC iota, PKC lambda, PKC mu, PKC nu, PKC theta, PKC zeta and Pkc1.

Also see PKC alpha Inhibitors, PKC alpha Activators, PKC alpha Substrates or PKC alpha Substrates for functional analysis of cellular responses to PKC alpha and PKC alpha.


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 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
p-PKC α Antibody (Ser 657) sc-12356 200 µg/ml $279
p-PKC α (Ser 657) P sc-12356 P
(peptide)
 100 µg/0.5 ml $61
p-PKC α Antibody (Ser 657)-R sc-12356-R 200 µg/ml $279
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
HeLa + PMA nuclear extract sc-2121 250 µg/0.05 ml $143
NIH/3T3 Whole Cell Lysate sc-2210 500 µg/200 µl $104
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human PRKCA 5578 17q24.2 NM_002737 P17252
 176960
Mouse Prkca 18750 11 E1 P20444
 N/A
 

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function (1,2). PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids (3). Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC (4,5). PKCs can be subdivided into at least two major classes, including conventional (c) PKC isoforms (å, ∫I, ∫II and ©) and novel (n) PKC isoforms (∂, é , Ω, h and †) (6). PKC isoforms can be activated through tyrosine phosphorylation and catalytically activated upon treatment with H2O2 (7,8). The Tyr 155, 525, 523 and 565 residues in the catalytic domain are crucial for activation of these enzymes (7,8). The residue Ser 643 appears to be an autophosphorylation site (9).

p-PKC α Antibody (Ser 657) Product Citations
See how others have used p-PKC α Antibody (Ser 657) and or p-PKC α Antibody (Ser 657) conjugates.


21 total citations
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p-PKC α Antibody (Ser 657) Data
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sc-12356
Western blot detection of PKC a phosphorylation using HeLa nuclear extracts treated with PMA. Blots were probed with PKC a (H-7): sc-8393 (A) and PKC a (Ser 657)-R: sc-12356-R (B,C). Antibody was preincubated with cognate non-phosphorylated (B) or phosphorylated (C) peptide.
sc-12356
Western blot analysis of l phosphatase-treated (A,C) and untreated (B,D) active human recombinant PKC α. Antibodies tested include PKC (MC5): sc-80 (A,B) and p-PKC α (Ser 657): sc-12356 (C,D).
sc-12356
p-PKC α (Ser 657): sc-12356. Immunofluorescence staining of methanol-fixed PMA-treated HeLa cells showing cytoplasmic localization.
sc-12356
p-PKC α (Ser 657): sc-12356. Western blot analysis of PKC α phosphorylation in NIH/3T3 whole cell lysate.
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