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home » Transcription Regulators » Smad Antibody / Smad Antibodies » p-Smad1/5/8 (Ser 463/Ser 465): sc-12353

p-Smad1/5/8 (Ser 463/Ser 465) Antibody: sc-12353

  |  Datasheet
  • goat polyclonal IgG, 200 µg/ml; also available as rabbit IgG, 200 µg/ml, sc-12353-R
  • epitope corresponding to a short amino acid sequence containing phosphorylated Ser 463 and Ser 465 of Smad1 of human origin
  • recommended for detection of Ser 463 and Ser 465 phosphorylated Smad1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
  • blocking peptide, sc-12353 P
  • TransCruz reagent for Gel Supershift and ChIP applications sc-12353 X, 200 µg/0.1 ml
 
Additional Smad Antibodies ...
 
Ordering InformationProduct Citations
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   Gel Shift   ChIP  
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human SMAD1 4086 4q31.22 NM_001003688, NM_005900 Q15797
 601595
Mouse Smad1 17125 8 C2 P70340
 N/A
 
Set Currency

 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
p-Smad1/5/8 (Ser 463/Ser 465) sc-12353 200 µg/ml $279
p-Smad1/5/8 (Ser 463/Ser 465) P sc-12353 P
(peptide)
 100 µg/0.5 ml $61
p-Smad1/5/8 (Ser 463/Ser 465) X sc-12353 X 200 µg/0.1 ml $279
p-Smad1/5/8 (Ser 463/Ser 465)-R sc-12353-R 200 µg/ml $279
Lambda Phosphatase sc-200312 100,000 units $462
Lambda Phosphatase sc-200312A 20,000 units $116

Smad1 Background Information
Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad), function downstream of TGF∫ receptor serine/threonine kinases and undergo serine phosphorylation in response to receptor activation (1,2). Following BMP (bone morphogenic protein) or TGF∫ binding to the targeted surface receptors, Smad1 (also designated Madr1 or JV4-1) becomes phosphorylated at Ser-463 and Ser-465 (3–5). Activated Smad1 associates with Smad4 and translocates to the nucleus, where the Smad1-Smad4 complex recruits DNA-binding proteins to activate specific gene transcription (3,4). BMP binding induces phosphorylation of Smad1, which enhances the binding of Smad1 to CBP to stimulate Smad1-dependent transcription (1,2). In addition, ALK1 and ALK2 specifically phosphorylate Smad1 on residues located in the MH2 domain and induces Smad1-dependent pathways (6,7).

p-Smad1/5/8 (Ser 463/Ser 465) Product Citations
See how others have used p-Smad1/5/8 (Ser 463/Ser 465): sc-12353 antibody and or p-Smad1/5/8 (Ser 463/Ser 465) antibody conjugates.


6 total citations
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p-Smad1/5/8 (Ser 463/Ser 465)
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sc-12353
p-Smad1/5/8 (Ser 463/Ser 465)-R: sc-12353-R. Western blot analysis of Smad1 phosphorylation in untreated (A), PMA-treated (B) and PMA and lambda protein phosphatase treated (C) HEK293 whole cell lysates.
sc-12353
p-Smad1/5/8 (Ser 463/Ser 465)-R: sc-12353-R. Western blot analysis of Smad1 phosphorylation in non-transfected: sc-117752 (A) and human Smad1 transfected: sc-171449 (B) 293T whole cell lysates.
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