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p-EGFR (Tyr 1173) Antibody: sc-12351 |
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- goat polyclonal IgG, 200 µg/ml; also available as rabbit IgG, 200 µg/ml, sc-12351-R
- recommended for detection of EGFR of mouse, rat and human origin phosphorylated at Tyr 1173 by WB, IP, IF and ELISA
- blocking peptide, sc-12351 P; also reactive with additional species, including equine, canine, bovine, porcine and avian
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EGFR Background Information
Epidermal growth factor mediates its effects on cell growth through its inter-action with a cell surface glycoprotein designated the EGF receptor. Binding of EGF or TGF alpha to the EGF receptor activates tyrosine-specific protein kinase activity intrinsic to the EGF receptor. The carboxy terminal tyrosine residues on EGFR, Tyr 1068 and Tyr 1173, are the major sites of autophosphorylation, which occurs as a result of EGF binding. Once activated, EGFR mediates the binding of the phosphotyrosine binding (PTB) domain of GRB2 through direct interactions with Tyr 1068 and Tyr 1086 and through indirect interactions with Tyr 1173 in the Ras signaling pathway. Tyr 1173 of EGFR also functions as a kinase substrate. Phosphorylation of Tyr 992, Tyr 1068 and Tyr 1086 is required for conformational change in the C-terminal tail of the EGF receptor.
| p-EGFR (Tyr 1173) Product Citations |
See how others have used p-EGFR (Tyr 1173): sc-12351 antibody and or p-EGFR (Tyr 1173) antibody conjugates.
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p-EGFR (Tyr 1173)
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Western blot analysis of EGFR expression in control (A,C) and EGF-treated A-431 (B,D) cultures. Antibodies tested include p-EGFR (Tyr 1173)-R: sc-12351-R (A,B) and EGFR (1005): sc-03 (C,D). p-EGFR (Tyr 1173): sc-12351. Western blot analysis of EGFR phosphorylation in untreated (A) and EGF-stimulated (B) A-431 cells. EGFR levels in the same untreated (C) and treated (D) cultures were determined by using EGFR (1005): sc-03. p-EGFR (Tyr 1173): sc-12351. Immunofluorescence staining of EGF-induced A-431 cells showing membrane localization (A), primary antibody absorbed with corresponding phosphorylated peptide showing no staining (B) and primary antibody absorbed with non-phosphorylated peptide (C).
p-EGFR (Tyr 1173)-R: sc-12351-R. Western blot analysis of EGFR phosphorylation in untreated (A) and lambda protein phosphatase treated (B) A-431 whole cell lysates.
p-EGFR (Tyr 1173): sc-12351. Western blot analysis of EGFR phosphorylation in untreated (A) and lambda protein phosphatase treated (B) A-431 whole cell lysates.
p-EGFR (Tyr 1173)-R: sc-12351-R. Western blot analysis of EGFR phosphorylation in untreated (A), EGF treated (B) and EGF and lambda protein phosphatase treated (C) A-431 whole cell lysates.
p-EGFR (Tyr 1173): sc-12351. Western blot analysis of EGFR phosphorylation in untreated (A), EGF treated (B) and EGF and lambda protein phosphatase treated (C) A-431 whole cell lysates.
Western blot analysis of EGFR phosphorylation in untreated (A, D), EGF treated (B, E) and EGF and lambda protein phosphatase treated (C, F) A-431 whole cell lysates. Antibodies tested include p-EGFR (Tyr 1173)-R: sc-12351-R (A, B, C) and EGFR (1005): sc-03 (D, E, F). Western blot analysis of EGFR phosphorylation in untreated (A, D), EGF treated (B, E) and EGF and lambda protein phosphatase treated (C, F) A-431 whole cell lysates. Antibodies tested include p-EGFR (Tyr 1173): sc-12351 (A, B, C) and EGFR (1005): sc-03 (D, E, F). Western blot analysis of EGFR phosphorylation in untreated (A, D), EGF treated (B, E) and EGF and lambda protein phosphatase treated (C, F) A-431 whole cell lysates. Antibodies tested include p-EGFR (Tyr 1173)-R: sc-12351-R (A, B, C) and EGFR (1005): sc-03 (D, E, F). |
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