p-Cdc2 p34 (Thr 14/Tyr 15)-R Antibody sc-12340-R

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p-Cdc2 p34 (Thr 14/Tyr 15)-R Antibody: sc-12340-R	\| Datasheet

rabbit polyclonal IgG, 200 µg/ml
recommended for detection of Cdc2 p34 of mouse, rat and human origin phosphorylated at Thr 14 and Tyr 15 and correspondingly phosphorylated Cdk2 and Cdk3 by WB, IP, IF and ELISA
blocking peptide, sc-12340 P; also reactive with additional species, including equine, canine, bovine, porcine and avian

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Product Name	Catalog #	Unit	Price
p-Cdc2 p34 (Thr 14/Tyr 15) P	sc-12340 P (peptide)	100 µg/0.5 ml	$61
p-Cdc2 p34 (Thr 14/Tyr 15)-R	sc-12340-R	200 µg/ml	$279
Lambda Phosphatase	sc-200312	100,000 units	$462
Lambda Phosphatase	sc-200312A	20,000 units	$116

WB Positive Control Cell Lysates (click product name for more information)

Product Name	Catalog #	Unit	Price
Saos-2 + hydroxyurea Cell Lysate	sc-2286	500 µg/200 µl	$104
HeLa Whole Cell Lysate	sc-2200	500 µg/200 µl	$104
K-562 Whole Cell Lysate	sc-2203	500 µg/200 µl	$104
HeLa + hydroxyurea Cell Lysate	sc-24682	500 µg/200 µl	$104
NAMALWA Cell Lysate	sc-2234	500 µg/200 µl	$104
Saos-2 Cell Lysate	sc-2235	500 µg/200 µl	$104

Cdc2 p34 Background Information
Cdc2, an evolutionarily conserved serine/threonine-specific protein kinase, is essential in the cell cycle transition from G2 to M phase. Cdc2 is regulated by association with B-type cyclins and by reversible phosophorylation. Cyclin B binding facilitates the phosphorylation of Cdc2 p34 on three regulatory sites: threonine 14, tyrosine 15, and threonine 161. In higher eukaryotes, Cdc2 is negatively regulated by phosphorylation of two residues located in the ATP-binding site, Thr 14 and Tyr 15. Cdc2 is positively regulated by the cyclin-dependent phosphorylation of Thr 161. Both phosphorylation and de- phosphorylation at Thr 161 are required for progression through the cell cycle.

p-Cdc2 p34 (Thr 14/Tyr 15)-R Product Citations

See how others have used p-Cdc2 p34 (Thr 14/Tyr 15)-R: sc-12340-R antibody and or p-Cdc2 p34 (Thr 14/Tyr 15)-R antibody conjugates.

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p-Cdc2 p34 (Thr 14/Tyr 15)-R

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Western blot analysis of Cdc2 p34 phosphorylation expression in HeLa whole cell lysates (A-C). Blots were probed with p-Cdc2 p34 (Thr 14/Tyr 15)-R: sc-12340-R preincubated with cognate phosphorylated (A) or cognate unphosphorylated (B) peptide and Cdc2 p34 (H-297): sc-747 (C).

p-Cdc2 p34 (Thr 14/Tyr 15)-R: sc-12340-R. Western blot analysis of phosphorylated Cdc2 p34 expression in HeLa (A) and hydroxyurea-treated HeLa (B) whole cell lysates.

p-Cdc2 p34 (Thr 14/Tyr 15)-R: sc-12340-R. Western blot analysis of Cdc2 p34 phosphorylation in non-transfected 293: sc-110760 (A), human Cdc2 p34 transfected 293: sc-128282 (B) and HeLa (C) whole cell lysates.

p-Cdc2 p34 (Thr 14/Tyr 15)-R: sc-12340-R. Western blot analysis of Cdc2 p34 phosphorylation in non-transfected 293T: sc-117752 (A), mouse Cdc2 p34 transfected 293T: sc-119124 (B) and K-562 (C) whole cell lysates.

p-Cdc2 p34 (Thr 14/Tyr 15)-R: sc-12340-R. Western blot analysis of Cdc2 p34 phosphorylation in untreated (A) and lambda protein phosphatase treated (B) HeLa whole cell lysates.

Western blot analysis of Cdc2 p34 phosphorylation in untreated (A, C) and lambda protein phosphatase treated (B, D) Saos-2 whole cell lysates. Antibodies tested include p-Cdc2 p34 (Thr 14/Tyr 15)-R: sc-12340-R (A, B) and Cdc2 p34 (PSTAIRE): sc-53 (C, D).

p-Cdc2 p34 (Thr 14/Tyr 15)-R: sc-12340-R. Western blot analysis of Cdc2 p34 phosphorylation in HeLa whole cell lysate.

Western blot analysis of Cdc2 p34 phosphorylation in untreated (A,F), Hydroxyurea treated (B,G), nocodazole treated (C,H), Hydroxyurea and lambda protein phosphatase treated (D,I) and nocodazole and lambda protein phosphatase treated (E,J) HeLa whole cell lysates. Antibodies tested include p-Cdc2 p34 (Thr 14/Tyr 15)-R: sc-12340-R (A-E) and p-Cdc2 p34 (PSTAIRE): sc-53 (F-J).

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p-Cdc2 p34 (Thr 14/Tyr 15)-R Antibody: sc-12340-R