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- goat polyclonal IgG, 200 µg/ml
- epitope mapping at the N-terminus of CGBP of human origin
- recommended for detection of CGBP of mouse, rat and human origin by WB, IP, IF, ELISA and indirect FCM using FITC-conjugated donkey anti-goat IgG: sc-2024; also reactive with additional species, including equine, canine, bovine and porcine
- blocking peptide, sc-12336 P
- TransCruz reagent for Gel Supershift and ChIP applications, sc-12336 X, 200 µg/0.1 ml
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Ordering Information
Recommended Support Products:
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| Species |
Gene Name |
Gene ID |
Chromosome Location |
Isoform (mRNA) Accession # |
Protein Accession # |
OMIM™ Number |
| Human |
CXXC1 |
30827 |
18q21.1 |
NM_014593 |
Q9P0U4
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609150 |
| Mouse |
Cxxc1 |
74322 |
18 E2 |
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Q9CWW7
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N/A |
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CGBP Background Information
The human genome contains discrete clusters of unmethylated CpG dinucleotides, called CpG islands, which contribute to the modulation of gene expression by binding transcription factors (1-4). Human CpG binding protein (CGBP), detected in K562 cells, is a widely expressed member of CpG binding proteins that requires the CpG dinucleotide to bind DNA (1). CGBP binds specifically to unmethylated CpG motifs and functions as a transcriptional activator (1). The CXXC domain of CGBP is conserved in DNA methyltransferase, human trithorax, and methyl-CpG binding protein (MBP), and is involved in DNA-binding (1,5,6). CGBP also contains several domains implicated in protein-protein interactions, such as a coiled-coil domain, and two PHD finger domains, the function of which remains to be determined (1). |
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CGBP (N-21)
Click on image to enlarge
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Western blot analysis of CGBP expression in Jurkat (A,C) and K-562 (B,D) nuclear extracts. Antibodies tested include CGBP (N-21): sc-12336 (A,B) and CGBP (C-20): sc-12339 (C,D). CGBP (N-21): sc-12336. Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear staining.
CGBP (N-21): sc-12336. Western blot analysis of CGBP expression in NIH/3T3 nuclear extract.
CGBP (N-21): sc-12336. Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with CGBP (N-21) followed by FITC-conjugated donkey anti-goat IgG: sc-2024. Black line histogram represents the isotype control, normal goat IgG: sc-3887. CGBP (N-21): sc-12336. Western blot analysis of CGBP expression in non-transfected: sc-117752 (A) and mouse CGBP transfected: sc-119204 (B) 293T whole cell lysates. |
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