Cdc14 Background Information
Cell cycle progression is controlled at a point late in G1 designated Start. The key cell cycle transitions in Saccharomyces cerevisiae are G1 to S, metaphase to anaphase, and the exit from mitosis, all of which are regulated by a complex network of proteins (1). The specific set of proteins required for the exit from mitosis include Tem1, Lte1, Cdc15, Dbf2/Dbf20, Cdc5, Mob1, and Cdc14 (1-2). Cdc14 is a dual specificity protein phosphatase that inactivates mitotic cyclin-dependent kinases (Cdks) (1,3-5). It is tethered to the nucleolus by the action of Net1, but is released in late anaphase/telophase by Tem1, a GTP-binding protein (1,2,6). Mutations in these genes arrest cells in late anaphase/telophase, which indicates that Cdc14 and Tem1 are necessary for the termination of the M phase in the cell cycle (1).
