CD64 Antibody (10.1): sc-1184

CD64 Antibody (10.1) is a mouse monoclonal IgG1 kappa light chain antibody that detects CD64 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). CD64 (10.1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD64, also known as FCGR1A, FcγRI, or high-affinity Fc-gamma receptor, is a surface glycoprotein that plays a critical role in the immune response by binding monomeric immunoglobulin G (IgG) with high affinity. CD64 is predominantly expressed on monocytes and macrophages, and expression can be induced in neutrophils following stimulation with interferon-gamma. CD64 functions as a mediator of phagocytosis and cell-mediated cytotoxicity, which are essential processes for pathogen clearance and immune response regulation. CD64 has been shown to interact with various signaling molecules, including Src family kinases such as Hck and Lyn, which enhance signaling capabilities upon receptor cross-linking. This interaction activates immune cells and subsequent immune response, highlighting CD64′s significance in both innate and adaptive immunity.

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CD64 Antibody (10.1) References:

1. Novel Fc gamma receptor I family gene products in human mononuclear cells.  |  Porges, AJ., et al. 1992. J Clin Invest. 90: 2102-9. PMID: 1430234
2. CD64 expression by neutrophil granulocytes.  |  Sack, U. 2017. Cytometry B Clin Cytom. 92: 189-191. PMID: 25522066
3. CD66b-CD64dimCD115- cells in the human bone marrow represent neutrophil-committed progenitors.  |  Calzetti, F., et al. 2022. Nat Immunol. 23: 679-691. PMID: 35484408
4. iPsc-derived natural killer cells expressing the FcγR fusion CD64/16A can be armed with antibodies for multitumor antigen targeting.  |  Snyder, KM., et al. 2023. J Immunother Cancer. 11: PMID: 38056893
5. CD64 plays a key role in diabetic wound healing.  |  Zhang, X., et al. 2024. Front Immunol. 15: 1322256. PMID: 38524127
6. P75NTR+CD64+ neutrophils promote sepsis-induced acute lung injury.  |  Fu, D., et al. 2024. Clin Immunol. 263: 110206. PMID: 38599263
7. CD64+ fibroblast-targeted vilanterol and a STING agonist augment CLDN18.2 BiTEs efficacy against pancreatic cancer by reducing desmoplasia and enriching stem-like CD8+ T cells.  |  Zhou, T., et al. 2024. Gut. 73: 1984-1998. PMID: 39187291
8. Activation-dependent expression of low affinity IgG receptors Fc gamma RII(CD32) and Fc gamma RIII(CD16) in subpopulations of human T lymphocytes.  |  Engelhardt, W., et al. 1995. Immunobiology. 192: 297-320. PMID: 7649565
9. IL-10 up-regulates human monocyte phagocytosis in the presence of IL-4 and IFN-gamma.  |  Capsoni, F., et al. 1995. J Leukoc Biol. 58: 351-8. PMID: 7665991
10. Multiple regions of human Fc gamma RII (CD32) contribute to the binding of IgG.  |  Hulett, MD., et al. 1995. J Biol Chem. 270: 21188-94. PMID: 7673151
11. Involvement of the high-affinity receptor for IgG (Fc gamma RI; CD64) in enhanced tumor cell cytotoxicity of neutrophils during granulocyte colony-stimulating factor therapy.  |  Valerius, T., et al. 1993. Blood. 82: 931-9. PMID: 7687898
12. Physical and functional association of the high affinity immunoglobulin G receptor (Fc gamma RI) with the kinases Hck and Lyn.  |  Wang, AV., et al. 1994. J Exp Med. 180: 1165-70. PMID: 8064233

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD64 Antibody (10.1) has 23 citations in a variety of scientific publications.

Primary antibodies like Anti-CD64 Antibody (10.1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD64 Antibody (10.1) Specifications

Alternate Product Name: Fc-gamma receptor 1

Product Clone Name: 10.1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: CD64

Epitope Species: human

Product Reactivity/Detection: CD64 cell surface marker

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 23 citations in various scientific publications

Available Conjugates/Forms for CD64 Antibody (10.1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CD64 antibody is CD64 Antibody (C-6): sc-515431.