CD45RO Antibody (UCH-L1): sc-1183

CD45RO Antibody (UCH-L1) is a mouse monoclonal IgG2a antibody engineered to target the CD45RO isoform of the CD45 protein, also known as Protein Tyrosine Phosphatase Receptor Type C (PTPRC). CD45RO is a crucial transmembrane glycoprotein expressed predominantly on memory T lymphocytes in humans, as well as on various hematopoietic cells in mice and rats, playing a vital role in regulating immune cell signaling and activation. Structurally, CD45RO consists of an extracellular domain generated through alternative splicing of exons 4, 5, and 6, resulting in a shorter extracellular region compared to other isoforms like CD45RA. This structural variability allows CD45RO to interact differently with other cell surface molecules, thereby modulating essential signal transduction pathways involved in T cell receptor (TCR) signaling. Located on the cell membrane, CD45RO functions as a phosphotyrosine phosphatase, dephosphorylating key signaling proteins such as Src family kinases Lck and Fyn, as well as Syk family kinases like ZAP-70. These interactions are pivotal for initiating and sustaining signaling cascades that drive T cell activation, proliferation, and differentiation, which are critical for maintaining immune system homeostasis and effective immune responses. Additionally, CD45RO undergoes post-translational modifications, including extensive glycosylation, which are essential for its stability, proper membrane localization, and interactions with other signaling proteins, thereby enhancing its regulatory functions. Anti-CD45RO antibody (UCH-L1) is raised against cultured human T cell line CA-1 and is effective in various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM) across human, mouse, and rat samples. By binding to CD45RO, CD45RO monoclonal antibody (UCH-L1) facilitates comprehensive analysis of memory T cell populations and their interactions with key signaling proteins, providing valuable insights into immune system dynamics and the mechanisms underlying immune-related diseases.

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CD45RO Antibody (UCH-L1) References:

1. Sialic acid-dependent epitopes of CD45 molecules of restricted cellular expression.  |  Bazil, V., et al. 1989. Immunogenetics. 29: 202-5. PMID: 2466778
2. The leukocyte common antigen family.  |  Thomas, ML. 1989. Annu Rev Immunol. 7: 339-69. PMID: 2523715
3. Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102.  |  Bazil, V., et al. 1989. Folia Biol (Praha). 35: 289-97. PMID: 2534480
4. Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes.  |  Woollett, GR., et al. 1985. Eur J Immunol. 15: 168-73. PMID: 2578966
5. Differential usage of three exons generates at least five different mRNAs encoding human leukocyte common antigens.  |  Streuli, M., et al. 1987. J Exp Med. 166: 1548-66. PMID: 2824653
6. Monoclonal antibodies (MT1, MT2, MB1, MB2, MB3) reactive with leukocyte subsets in paraffin-embedded tissue sections.  |  Poppema, S., et al. 1987. Am J Pathol. 127: 418-29. PMID: 3296769
7. The demonstration of B-cell, T-cell and myeloid antigens in paraffin sections.  |  West, KP., et al. 1986. J Pathol. 150: 89-101. PMID: 3540244
8. New marker of B lymphocytes, MB2: comparison with other lymphocyte subset markers active in conventionally processed tissue sections.  |  Hall, PA., et al. 1987. J Clin Pathol. 40: 151-6. PMID: 3546393
9. Tetraspanin CD53 controls T cell immunity through regulation of CD45RO stability, mobility, and function.  |  Dunlock, VE., et al. 2022. Cell Rep. 39: 111006. PMID: 35767951
10. CD45RA and CD45RO Are Regulated in a Cell-Type Specific Manner in Inflammation and Sepsis.  |  Ahmed, MGT., et al. 2023. Cells. 12: PMID: 37508538
11. CD45RO expression on peripheral lymphocytes as a prognostic marker for adult T-cell leukemia.  |  Suzuki, M., et al. 1998. Leuk Lymphoma. 28: 583-90. PMID: 9613989

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD45RO Antibody (UCH-L1) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-CD45RO Antibody (UCH-L1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD45RO Antibody (UCH-L1) Specifications

Alternate Product Name: CD45

Product Clone Name: UCH-L1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: CD45RO

Epitope Species: human

Product Reactivity/Detection: CD45RO (180-185 kDa)

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for CD45RO Antibody (UCH-L1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, APC: Allophycocyanin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CD45 antibody is CD45 Antibody (35-Z6): sc-1178.