goat polyclonal IgG, 200 µg/ml; also available as rabbit IgG, 200 µg/ml, sc-11761-R
epitope corresponding to a short amino acid sequence containing phosphorylated Tyr 694 of Stat5a of human origin
recommended for detection of Tyr 694 phosphorylated Stat5a and Tyr 699 phosphorylated Stat5b of mouse, rat and human origin by WB, IF and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
blocking peptide, sc-11761 P
TransCruz reagent for Gel Supershift and ChIP applications sc-11761 X, 200 µg/0.1 ml
Stat5 Background Information Stat5 (Signal Transducers and Activators of Transcription 5) is important in regulating T cell functions involving the receptors for Interleukin-2 (IL-2) (1). IL-2 stimulates the rapid phosphorylation of both serine and tyrosine residues of Stat5a and Stat5b in human T lymphocytes and in several IL-2-responsive lymphocytic cell lines (2–4). IL-2 differentially induces serine phosphorylation of Stat5a and Stat5b on Ser726 and Ser731, respectively (2,5). Stat5b is preferentially phosphorylated and displays more protracted serine phosphorylation kinetics than Stat5a (2,5). Both the acid-rich region and the COOH terminus of IL-2Rb can independently mediate IL-2-induced Stat 5a/b serine phosphorylation, suggesting that Stat5a/b serine phosphorylation occurs at a postreceptor level (2). Stat5a is phosphorylated on Tyr694 in a prolactin-sensitive manner, whereas serine phosphorylation is constitutive. Activation of Stat5 by IL-2 may help govern the biological effects of IL-2 during the immune response (3). Ser779 is constitutively phosphorylated in the mammary gland, and Ser725 phosphorylation influences prolactin-stimulated in vitro DNA binding activity.
p-Stat5 (Tyr 694/Tyr 699) Product Citations
See how others have used p-Stat5 (Tyr 694/Tyr 699): sc-11761 antibody and or p-Stat5 (Tyr 694/Tyr 699) antibody conjugates.
