4E-BP1 (h): 293T Lysate sc-116590

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4E-BP1 (h): 293T Lysate: sc-116590	\| Datasheet

100 µg protein in 200 µl SDS-PAGE buffer; suitable as Western Blotting control for 4E-BP1 antibodies
human 4E-BP1 transfected 293T lysate
suitable control antibody: 4E-BP1 (C-19): sc-6024
should be stored at -20°C and repeated freezing and thawing should be minimized
sample vial should be boiled once prior to use
non-transfected control 293T lysate: sc-117752

Species	Gene Name	Gene ID	Chromosome Location	Protein Accession #	OMIM™ Number

Human	EIF4EBP1	1978	8p12	Q13541	602223

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Ordering Information

Product Name	Catalog #	Unit	Price	Qty	Add	Favorites
4E-BP1 (h): 293T Lysate	sc-116590	100µg/200µl	$205

Recommended Control Antibody (click product name for more information)

Product Name	Catalog #	Unit	Price	Qty	Add	Favorites
4E-BP1 (C-19)	sc-6024	200 µg/ml	$279
donkey anti-goat IgG-HRP	sc-2020	200 µg/0.5 ml	$70

4E-BP1 (h): 293T Lysate

Click on image to enlarge

4E-BP1 (R-113): sc-6936. Western blot analysis of 4E-BP1 expression in non-transfected: sc-117752 (A) and human 4E-BP1 transfected: sc-116590 (B) 293T whole cell lysates.

4E-BP1 (P-1): sc-9977. Western blot analysis of 4E-BP1 expression in non-transfected: sc-117752 (A) and human 4E-BP1 transfected: sc-116590 (B) 293T whole cell lysates.

4E-BP1 (C-19): sc-6024. Western blot analysis of 4E-BP1 expression in non-transfected: sc-117752 (A) and human 4E-BP1 transfected: sc-116590 (B) 293T whole cell lysates.

4E-BP1 (N-19): sc-6025. Western blot analysis of 4E-BP1 expression in non-transfected: sc-117752 (A) and human 4E-BP1 transfected: sc-116590 (B) 293T whole cell lysates.

p-4E-BP1 (Ser 65)-R: sc-18091-R. Western blot analysis of 4E-BP1 phosphorylation in non-transfected: sc-117752 (A) and human 4E-BP1 transfected: sc-116590 (B) 293T whole cell lysates.

p-4E-BP1 (Thr 70)-R: sc-23768-R. Western blot analysis of 4E-BP1 phosphorylation in non-transfected: sc-117752 (A) and human 4E-BP1 transfected: sc-116590 (B) 293T whole cell lysates.

4E-BP1 (11G12C11): sc-81149. Western blot analysis of 4E-BP1 expression in non-transfected: sc-117752 (A) and human 4E-BP1 transfected: sc-116590 (B) 293T whole cell lysates.

p-4E-BP1/2/3 (Thr 45): sc-101625. Western blot analysis of 4E-BP1 phosphorylation in non-transfected: sc-117752 (A), untreated human 4E-BP1 transfected: sc-116590 (B) and lambda protein phosphatase treated human 4E-BP1 transfected: sc-116590 (C) 293T whole cell lysates.

p-4E-BP1 (Ser 65/Thr 70): sc-12884. Western blot analysis of 4E-BP1 phosphorylation in non-transfected: sc-117752 (A), untreated human 4E-BP1 transfected: sc-116590 (B), lambda protein phosphatase treated non-transfected: sc-117752 (C) and lambda protein phosphatase treated human 4E-BP1 transfected: sc-116590 (D) 293T whole cell lysates.

p-4E-BP1 (Thr 36)-R: sc-18080-R. Western blot analysis of 4E-BP1 phosphorylation in non-transfected: sc-117752 (A), untreated human 4E-BP1 transfected: sc-116590 (B) and lambda protein phosphatase treated human 4E-BP1 transfected: sc-116590 (C) 293T whole cell lysates.

p-4E-BP1 (Thr 70)-R: sc-18092-R. Western blot analysis of 4E-BP1 phosphorylation in non-transfected: sc-117752 (A), untreated human 4E-BP1 transfected: sc-116590 (B) and lambda protein phosphatase treated human 4E-BP1 transfected: sc-116590 (C) 293T whole cell lysates.

p-4E-BP1 (Ser 65/Thr 70)-R: sc-12884-R. Western blot analysis of 4E-BP1 phosphorylation in non-transfected: sc-117752 (A), untreated human 4E-BP1 transfected: sc-116590 (B) and lambda protein phosphatase treated human 4E-BP1 transfected: sc-116590 (C) 293T whole cell lysates.

p-4E-BP1/2/3 (Thr 45)-R: sc-18090-R. Western blot analysis of 4E-BP1 phosphorylation in non-transfected: sc-117752 (A), untreated human 4E-BP1 transfected: sc-116590 (B) and lambda protein phosphatase treated human 4E-BP1 transfected: sc- 116590 (C) 293T whole cell lysates.

Western blot analysis of 4E-BP1 phosphorylation in non-transfected: sc-117752 (A, D), untreated human 4E-BP1 transfected: sc-116590 (B, E) and lambda protein phosphatase treated human 4E-BP1 transfected: sc-116590 (C, F) 293T whole cell lysates. Antibodies tested include p-4E-BP1 (Ser 65/Thr 70): sc-12884 (A, B, C) and 4E-BP1 (R-113): sc-6936 (D, E, F).

Western blot analysis of 4E-BP1 phosphorylation in non-transfected: sc-117752 (A, D), untreated human 4E-BP1 transfected: sc-116590 (B, E) and lambda protein phosphatase treated human 4E-BP1 transfected: sc-116590 (C, F) 293T whole cell lysates. Antibodies tested include p-4E-BP1 (Thr 36)-R: sc-18080-R (A, B, C) and 4E-BP1 (R-113): sc-6936 (D, E, F).

Western blot analysis of 4E-BP1 phosphorylation in non-transfected: sc-117752 (A, D), untreated human 4E-BP1 transfected: sc-116590 (B, E) and lambda protein phosphatase treated human 4E-BP1 transfected: sc-116590 (C, F) 293T whole cell lysates. Antibodies tested include p-4E-BP1 (Thr 70)-R: sc-18092-R (A, B, C) and 4E-BP1 (R-113): sc-6936 (D, E, F).

Western blot analysis of 4E-BP1 phosphorylation in non-transfected: sc-117752 (A, D), untreated human 4E-BP1 transfected: sc-116590 (B, E) and lambda protein phosphatase treated human 4E-BP1 transfected: sc-116590 (C, F) 293T whole cell lysates. Antibodies tested include p-4E-BP1/2/3 (Thr 45): sc-101625 (A, B, C) and 4E-BP1 (R-113): sc-6936 (D, E, F).

4E-BP1 (P-1): sc-9977. Western blot analysis of 4E-BP1 expression in non-transfected: sc-117752 (A) and human 4E-BP1 transfected: sc-116590 (B) 293T whole cell lysates.

Western blot analysis of 4E-BP1 phosphorylation in non-transfected: sc-117752 (A,D), untreated human 4E-BP1 transfected: sc-116590 (B,E) and lambda protein phosphatase (sc-200312A) treated human 4E-BP1 transfected: sc-116590 (C,F) 293T whole cell lysates. Antibodies tested include p-4E-BP1/2/3 (Thr 45)-R: sc-18090-R (A,B,C) and 4E-BP1 (11G12C11): sc-81149 (D,E,F).

Western blot analysis of 4E-BP1 phosphorylation in non-transfected: sc-117752 (A,D), untreated human 4E-BP1 transfected: sc-116590 (B,E) and lambda protein phosphatase (sc-200312A) treated human 4E-BP1 transfected: sc-116590 (C,F) 293T whole cell lysates. Antibodies tested include p-4E-BP1/2/3 (A-10): sc-271947 (A,B,C) and 4E-BP1 (11G12C11): sc-81149 (D,E,F).

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4E-BP1 (h): 293T Lysate: sc-116590