CENP-F Background Information A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. Centromere Protein F, CENP-F (also designated mitosin) is a nuclear matrix kinetochore protein that plays a role in mitotic events. In HeLa cells, CENP-F gradually accumulates in the cell cycle, and like CENP-E is preferentially expressed during mitosis where it mediates the G(2) to M phase checkpoint. Upon completion of mitosis, CENP-F is rapidly degraded. CENP-F consists of two coil domains that flank a central flexible core and contains a P-loop (ADIPTGKT) nucleotide binding site in its globular carboxy terminus.
