epitope mapping at the C-terminus of CENP-B of human origin
recommended for detection of CENP-B of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
CENP-B Background Information A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. Both centromere proteins CENP-B and CENP-H are contained in the centromeric heterochromatin between kinetochores, and are involved in maintaining sister chromatid cohesion. The highly dispersed CENP-B promotes and maintains the joining of DNA satellites in the centromere. CENP-B targets centromeric a-DNA and protects it from digestion by nucleases as well as preventing DNAse or restriction enzyme digestion from affecting the morphology of centromeres. CENP-H contains a coiled-coil structure and a nuclear localization signal. CENP-H is specifically and constitutively localized to kinetochores and plays a role in the organization and function of kinetochores throughout the cell cycle.
CENP-B (C-16)
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CENP-B (C-16): sc-11282. Western blot analysis of CENP-B expression in non-transfected: sc-117752 (A) and human CENP-B transfected: sc-116535 (B) 293T whole cell lysates.
