epitope mapping within an internal region of CENP-A of human origin
recommended for detection of CENP-A of mouse, rat and human origin by WB, IF and ELISA; also reactive with additional species, including canine, bovine, porcine and avian
CENP-A Background Information A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. Centromere protein-A (CENP-A) is a Histone H3-like protein that contains a C-terminal H3-like domain, required for centro-mere localization of CENP-A, and an antigenic N-terminal domain. CENP-A, originally isolated from HeLa cells, is essential for kinetochore targeting of CENP-C. In the presence of DNA, CENP-A forms an octameric complex with histones H4, H2A and H2B. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes, on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on the chromosome.
