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Ets-1/Ets-2 (C-275) Antibody: sc-112 |
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- rabbit polyclonal IgG, 200 µg/ml
- epitope mapping within the highly conserved C-terminal domain of Ets-1/Ets-2 of human origin
- recommended for detection of Ets-1 and Ets-2 of mouse, rat, human, Drosophila, Xenopus and C. elegans origin by WB, IP and ELISA
; also reactive with additional species, including equine, canine, bovine, porcine and avian
- blocking peptide, sc-112 P
- TransCruz reagent for Gel Supershift and ChIP applications, sc-112 X, 200 µg/0.1 ml
- agarose conjugate for IP studies, sc-112 AC, 500 µg/0.25 ml agarose
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Ets-1/Ets-2 Background Information
Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2, Erg-1–3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF. Members of the Ets gene family exhibit varied patterns of tissue expression, and share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. This conserved domain is essential for Ets-1 binding to DNA and is likely to be responsible for the DNA-binding activity of all members of the Ets gene family. Several of these proteins have been shown to recognize similar motifs in DNA that share a centrally located 5’-GGAA-3’ element. Evidence indicates that the DNA binding activity by Ets-1 is regulated at the level of phosphorylation.
| Ets-1/Ets-2 (C-275) Product Citations |
See how others have used Ets-1/Ets-2 (C-275): sc-112 antibody and or Ets-1/Ets-2 (C-275) antibody conjugates.
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Ets-1/Ets-2 (C-275)
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Ets-1/Ets-2 (C-275): sc-112. Western blot analysis of Ets-1/Ets-2 expression in MM-142 (A), KNRK (B) and 3611-RF (C) nuclear extracts.
ChiP analysis of transcription factor binding to the CD28 responsive positive regulatory region IV (PRRIV) in resting (A,B) and CD3+CD28-stimulated human primary T cells. 10% (A,C) and 1% (B,D) of input DNAs were amplified. Antibodies used included c-Jun (D): sc-44; c-Fos (6-2H-2F): sc-447 and Ets-1/Ets-2 (C-275): sc-112. Data kindly provided by J. Imbert and reproduced with permission from Yeh, J.H., et al, Mol. Cell. Biol., 2001, 21:4515-4527. Ets-1/Ets-2 (C-275): sc-112. Western blot analysis of Ets-2 expression in non-transfected 293T: sc-117752 (A), mouse Ets-2 transfected 293T: sc-120131 (B) and HeLa (C) whole cell lysates. Ets-1/Ets-2 (C-275): sc-112. Western blot analysis of Ets-1 expression in non-transfected: sc-117752 (A) and mouse Ets-1 transfected: sc-120130 (B) 293T whole cell lysates and CCRF-CEM nuclear extract (C). Ets-1/Ets-2 (C-275): sc-112. Western blot analysis of Erg-1/2/3 expression in non-transfected 293T: sc-117752 (A), human Erg-1/2/3 transfected 293T: sc-115805 (B) and Jurkat (C) whole cell lysates. Ets-1/Ets-2 (C-275): sc-112. Western blot analysis of Ets-1/Ets-2 expression in Jurkat nuclear extract.
Ets-1/Ets-2 (C-275): sc-112. Western blot analysis of Ets-2 expression in non-transfected: sc-117752 (A) and mouse Ets-2 transfected: sc-120131 (B) 293T whole cell lysates and KNRK nuclear extract (C). |
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