epitope mapping near the C-terminus of FIR of human origin
recommended for detection of FIR of mouse, rat and human origin by WB, IF and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
FIR Background Information Activation of FUSE, the far-upstream element, is required for the proper expression of the mammalian gene c-myc (1). The binding of FBP (FUSE-binding protein) to FUSE is necessary for c-myc expression (2,3). The FBP interacting repressor, FIR, binds to the central DNA-binding domain of FBP and can serve as an overriding negative regulator of c-myc promoter activity (4). FIR interacts with the TFIIH complex, which is a multifunctional, multisubunit RNA polymerase II transcription factor that interacts with several DNA-binding transactivators (4-6). FIR blocks activator-dependent, but not basal transcription through TFIIH (4). FIR shares identity with seven in absentia (siah) binding protein 1 (4). FIR is expressed in spleen, thymus, prostate, small intestine, colon, and peripheral blood leukocytes, and with relatively higher levels of expression in testis and ovary (4).
