Control 293 Lysate: sc-110760
- 100 µg protein in 200 µl SDS-PAGE buffer
- Western Blot negative control lysate derived from non-transfected 293 cells
- Suitable for use with our 293 Transfected Lysates
- Recommended use: 10-20 µl per lane
- should be stored at -20° C and repeated freezing and thawing should be minimized
- sample vial should be boiled once prior to use
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293 Control Lysate is derived from the 293 cell line, a human embryonic kidney cell line widely used in molecular and cellular biology research. This lysate serves as a valuable reference sample in various experimental applications, providing a consistent and comprehensive mixture of cytoplasmic and nuclear proteins. The 293 cell line is known for its robust growth and high transfection efficiency, making it an ideal model for studying gene expression, protein interactions, and signal transduction pathways. In research, 293 Control Lysate is essential for establishing baselines and ensuring experimental accuracy. It is frequently used in Western blotting to verify the presence, size, and abundance of target proteins by serving as a positive control. Additionally, the lysate is employed in immunoprecipitation assays to test antibody specificity and in mass spectrometry for protein identification and quantification. Researchers also use it in electrophoretic mobility shift assays (EMSA) to analyze DNA-binding protein activities. The use of 293 Control Lysate allows for the standardization of experimental conditions, ensuring that observed differences are due to experimental variables rather than inconsistencies in sample preparation. The origin and quality of the 293 cell line are rigorously maintained, providing a reliable and reproducible tool for basic scientific research, particularly in studies involving cellular signaling, gene regulation, and protein function.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Control 293 Lysate | sc-110760 | 100 µg/200 µl | $118.00 |