p53R2 Background Information The p53 gene is a highly characterized tumor suppressor that is often inactivated in various human cancers. p53 is a transcription factor that mediates cell cycle arrest and apoptosis by binding to DNA and activating the transcription of specific genes. p53 is also thought to be involved in DNA repair by the transcriptional activation of a ribonucleotide reductase gene, p53R2, after exposure to genotoxic stresses. p53R2 displays a significant similarity to ribonucleotide reductase small subunit (R2), and the expression of R2 is elevated at the onset of the S-phase of the cell cycle. However, only p53R2 expression is induced in response to ultraviolet and g-irradiation and adriamycin treatment. p53R2 translocates to the nucleus upon DNA damage, and subsequently, supplies an immediate pool of dNTPs necessary for DNA repair.
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p53R2 (N-16)
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p53R2 (N-16): sc-10840. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining.
p53R2 siRNA (h): sc-36158. Western blot analysis of p53R2 expression in non-transfected control (A) and p53R2 siRNA transfected (B) HeLa cells. Blot probed with p53R2 (N-16): sc-10840. GAPDH (FL-335): sc-25778 used as specificity and loading control.
p53R2 (N-16): sc-10840. Western blot analysis of p53R2 expression in non-transfected: sc-117752 (A) and human p53R2 transfected: sc-111702 (B) 293T whole cell lysates.
p53R2 (N-16): sc-10840. Western blot analysis of p53 phosphorylation in untreated (A) and UV irradiated (B) HeLa whole cell lysates.
p53R2 (N-16): sc-10840. Western blot analysis of p53R2 expression in SW480 (A) and MCF7 (B) whole cell lysates.
