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- rabbit polyclonal IgG, 200 µg/ml
- epitope mapping near the C-terminus of E2F-4 of human origin
- recommended for detection of E2F-4 and, to a lesser extent, E2F-5 of mouse, rat and human origin by WB, IP, IF, ELISA and indirect FCM using PE (sc-3739)- and FITC (sc-2012)-conjugated goat anti-rabbit IgG; also reactive with additional species, including equine, canine, bovine, porcine and avian
- blocking peptide, sc-1082 P
- TransCruz reagent for Gel Supershift and ChIP applications, sc-1082 X, 200 µg/0.1 ml
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Ordering InformationProduct Citations
Recommended Support Products:
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| Species |
Gene Name |
Gene ID |
Chromosome Location |
Isoform (mRNA) Accession # |
Protein Accession # |
OMIM™ Number |
| Human |
E2F4 |
1874 |
16q22.1 |
NM_001950 |
Q16254
|
600659 |
| Mouse |
E2f4 |
104394 |
8 D3 |
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Q8R0K9
|
N/A |
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E2F-4 Background Information
The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcription regulators including E2F-2, E2F-3, E2F-4, E2F-5, E2F-6 and E2F-7 each of which forms heterodimers with a second protein, DP-1, forming an “active” E2F transcriptional regulatory complex.
| E2F-4 (A-20) Product Citations |
See how others have used E2F-4 (A-20): sc-1082 antibody and or E2F-4 (A-20) antibody conjugates.
49 total citations Loading citations.
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E2F-4 (A-20)
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E2F-4 (A-20): sc-1082. Immunofluorescence staining of methanol-fixed Jurkat cells showing nuclear staining.
E2F-4 (A-20): sc-1082. Western blot analysis of E2F-4 expression in Jurkat (A) and Y79 (B) nuclear extracts.
ChIP analysis of c-Myc promoter occupancy in response to lithium stimulation in serum synchronized C2C12 cells. Antibodies tested include HDAC1 (H-15): sc-7872, HDAC1 (C-19): sc-6298 and HDAC1 (H-11): sc-8410 (A), β-catenin (H-120): sc-7199, β-catenin (C-18): sc-1496 and β-catenin (E-5): sc-7963 (B), E2F-4 (A-20): sc-1082, E2F-4 (D-3): sc-6851, E2F-4 (RK-13): sc-511 and E2F-4 (C-20): sc-866 (C), and p130 (C-20): sc-317, p130 (211.6): sc-9963 and p130 (H-125): sc-20678 (D). Data kindly provided by M.G. Rosenfeld and reproduced with permission from Proc. Natl. Acad. Sci. 100: 3245-3250. Copyright 2003 National Academy of Sciences, USA. E2F-4 (A-20): sc-1082. Western blot analysis of E2F-4 expression in non-transfected: sc-117752 (A) and mouse E2F-4 transfected: sc-119883 (B) 293T whole cell lysates. E2F-4 (A-20): sc-1082. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with E2F-4 (A-20), followed by FITC-conjugated goat anti-rabbit IgG: sc-2012. Black line histogram represents the isotype control, normal rabbit IgG: sc-3888. E2F-4 (A-20): sc-1082. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with E2F-4 (A-20), followed by FITC-conjugated goat anti-rabbit IgG: sc-2012. Black line histogram represents the isotype control, normal rabbit IgG: sc-3888. E2F-4 (A-20): sc-1082. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with E2F-4 (A-20), followed by FITC-conjugated chicken anti-rabbit IgG: sc-2990. Black line histogram represents the isotype control, normal rabbit IgG: sc-3888. E2F-4 (A-20): sc-1082. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with E2F-4 (A-20), followed by FITC-conjugated donkey anti-rabbit IgG: sc-2090. Black line histogram represents the isotype control, normal rabbit IgG: sc-3888. E2F-4 (A-20): sc-1082. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with E2F-4 (A-20), followed by PE-conjugated mouse anti-rabbit IgG: sc-3753. Black line histogram represents the isotype control, normal rabbit IgG: sc-3888. goat anti-rabbit IgG-PE: sc-3739. Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with E2F-4 (A-20), followed by PE-conjugated goat anti-rabbit IgG: sc-3739. Black line histogram represents the isotype control, normal rabbit IgG: sc-3888. Antibody tested:E2F-4 (A-20): sc-1082. |
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