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- rabbit polyclonal IgG, 200 µg/ml
- epitope mapping at the C-terminus of Stat5a of mouse origin
- recommended for detection of Stat5a of mouse, rat and human origin by WB, IP, IF, ELISA and indirect FCM using PE (sc-3739)- or FITC (sc-2012)-conjugated goat anti-rabbit IgG; non cross-reactive with Stat5b; also reactive with additional species, including equine and canine
- blocking peptide, sc-1081 P
- TransCruz reagent for Gel Supershift and ChIP applications, sc-1081 X, 200 µg/0.1 ml
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Ordering InformationProduct Citations
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| Species |
Gene Name |
Gene ID |
Chromosome Location |
Isoform (mRNA) Accession # |
Protein Accession # |
OMIM™ Number |
| Human |
STAT5A |
6776 |
17q21.2 |
NM_003152 |
P42229
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601511 |
| Mouse |
Stat5a |
20850 |
11 D |
|
P42230
|
N/A |
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Stat5a Background Information
Signal transducer and activator of transcription 5A (Stat5a) and Stat5b, which share 96% homology, undergo receptor tyrosine kinase or G protein-coupled receptor-dependent phosphorylation in response to cytokines or growth factors, and then form homo- or heterodimers that translocate to the nucleus, where they initiate transcription. Activation of Stat5a via IL-2, IL-3, IL-7/ GM-CSF, erythropoietin, thrombopoietin and growth hormones influences proliferation, differentiation and apoptosis in lymphohematopoietic cells. Phos-phorylation of Stat5a at Ser127/Ser128 and Ser779 are contigent on ErbB-4-mediated activation of Stat5a. Activation of Stat5b via IL-2, IL-4, CSF-1 and growth hormones influences TCR signaling, apoptosis, adult mammary gland development and sexual dimorphism of liver gene expression. Stat5b is the major liver-expressed Stat5 form that has been shown to fuse with the retin-oic acid receptor a gene in acute promyelocytic leukemias (APLL). Stat5a/b null mice have severely impaired lymphoid development and differentiation.
| Stat5a (L-20) Product Citations |
See how others have used Stat5a (L-20): sc-1081 antibody and or Stat5a (L-20) antibody conjugates.
88 total citations Loading citations.
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Stat5a (L-20)
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Stat5a (L-20): sc-1081. Western blot analysis of Stat5a expression in K-562 nuclear extract.
Stat5a (L-20): sc-1081. Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear staining.
Stat5a (L-20): sc-1081. Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with Stat5a (L-20), followed by FITC-conjugated goat anti-rabbit IgG: sc-2012. Black line histogram represents the isotype control, normal rabbit IgG: sc-3888. Stat5a (L-20): sc-1081. Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with Stat5a (L-20), followed by PE-conjugated goat anti-rabbit IgG: sc-3739. Black line histogram represents the isotype control, normal rabbit IgG: sc-3888. Stat5a (L-20): sc-1081. Western blot analysis of Stat5a expression in K-562 whole cell lysate.
Stat5a (L-20): sc-1081. Western blot analysis of Stat5a expression in non-transfected: sc-117752 (A) and human Stat5a transfected: sc-114013 (B) 293T whole cell lysates. Stat5a (L-20): sc-1081. Western blot analysis of Stat5a expression in non-transfected: sc-117752 (A) and human Stat5a transfected: sc-173719 (B) 293T whole cell lysates. Stat5a (L-20): sc-1081. Western blot analysis of Stat5a expression in non-transfected: sc-117752 (A) and human Stat5a transfected: sc-173609 (B) 293T whole cell lysates. |
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