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- rabbit polyclonal IgG, 200 µg/ml
- epitope corresponding to amino acids 139-347 of CCAAT-binding transcription factor subunit B (NF-YA) of human origin
- recommended for detection of NF-YA of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine, canine and porcine
- TransCruz reagent for Gel Supershift and ChIP applications, sc-10779 X, 200 µg/0.1 ml
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NF-YA Background Information
The CCAAT-binding factor NF-Y is a heteromeric transcription factor that specifically binds to CCAAT sequences in many eukaryotic genes. NF-Y is made up of three subunits, NF-YA, NF-YB, and NF-YC. All three components are necessary for DNA binding. In each NF-Y subunit, the segment needed for formation of the NF-Y-DNA complex is conserved from yeast to human. These conserved segments are homologous to the histone-fold motif of eukaryotic histones. The DNA binding domains of the NF-YB and NF-YC subunits have been suggested to interact through a protein-protein histone-fold “handshake” motif in a manner analogous to the histone proteins, H2B and H2A, respectively.
| NF-YA (H-209) Product Citations |
See how others have used NF-YA (H-209): sc-10779 antibody and or NF-YA (H-209) antibody conjugates.
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NF-YA (H-209)
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NF-YA (H-209): sc-10779. Western blot analysis of NF-YA expression in A-431 (A) and K-562 (B) whole cell lysates.
NF-YA (H-209): sc-10779. Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear localization.
NF-YA (H-209): sc-10779. Western blot analysis of NF-YA expression in non-transfected: sc-117752 (A) and mouse NF-YA transfected: sc-122034 (B) 293T whole cell lysates. |
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