 |
- rabbit polyclonal IgG, 200 µg/ml
- epitope corresponding to amino acids 209-296 mapping near the N-terminus of Brg-1 of human origin
- recommended for detection of Brg-1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including avian
- TransCruz reagent for Gel Supershift and ChIP applications, sc-10768 X, 200 µg/0.1 ml
|
|
| |
Ordering InformationProduct Citations
Recommended Support Products:
(click button of application of choice)
| |
| Species |
Gene Name |
Gene ID |
Chromosome Location |
Isoform (mRNA) Accession # |
Protein Accession # |
OMIM™ Number |
| Human |
SMARCA4 |
6597 |
19p13.2 |
NM_003072 |
P51532
|
603254 |
| Mouse |
Smarca4 |
20586 |
9 A3 |
|
NP_035547
|
N/A |
| |
Brg-1 Background Information
The SWI-SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF2å) and Brg-1 (also designated SNF2ß) are the ATPase subunits of the mammalian SWI-SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI-SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits are thought to play regulatory roles. hSNF2L and hSNF2H both appear to be homologs of Drosophila ISWI, a Brm related ATPase that is present in chromatin remodeling complexes other than SWI/SNF, including the NURF (nucleosome remodeling factor).
| Brg-1 (H-88) Product Citations |
See how others have used Brg-1 (H-88): sc-10768 antibody and or Brg-1 (H-88) antibody conjugates.
87 total citations Loading citations.
|
| |
|
|
|
Brg-1 (H-88)
Click on image to enlarge
|
|
Brg-1 (H-88): sc-10768. Western blot analysis of Brg-1 expression in HeLa (A) and K-562 (B) nuclear extracts.
Brg-1 (H-88): sc-10768. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung tumor showing nuclear staining.
ChIP analysis of recruitment of CBP, p300, PCA and BRG1 to the IL-2α (CD25) promoter in vivo. Antibodies tested include CBP (A-22): sc-369, p300 (N-15): sc-584, PCAF (H-369): sc-8999, and Brg-1 (H-88): sc-10768. CD3ε and Oct-2 promoter regions were employed as positive and negative controls, respectively. DNA was isolated from Rag -/- thymocytes or Rag -/- thymocytes expressing TCRβ. Data kindly provided by J. Imbert and reproduced from Yeh, J-H., et al., Nucleic Acids Research, 2002, 30: 1944-1951, with permission from Oxford University Press. Brg-1 (H-88): sc-10768. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
|
|
Download
|
|
