epitope mapping near the C-terminus of BRIDGE-1 of rat origin
recommended for detection of BRIDGE-1 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
blocking peptide, sc-10671 P
TransCruz reagent for Gel Supershift and ChIP applications, sc-10671 X, 200 µg/0.1ml
BRIDGE-1 Background Information BRIDGE-1, a protein homologous to a previously cloned proteasome subunit p27 is important in regulating Insulin and other islet genes in the pancreas. BRIDGE-1 is highly expressed in pancreatic b-cells and is predominantly located in the nucleus, although lower levels are expressed in the cytoplasm. BRIDGE-1 contains a conserved PDZ-like domain that mediates protein-protein interactions in a variety of intracellular signaling processes, including the transactivational activity of E2A. One mechanism of the activation of gene transcription in pancreatic b-cells is the interaction of E2A with coactivating proteins such as CBP, p300 and BRIDGE-1. The interaction of E12 and E47, members of the E2A family of transcription factors, with the PDZ-domain of BRIDGE-1 suggest a novel mechanism for Insulin gene regulation.
BRIDGE-1 (R-17)
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BRIDGE-1 (R-17): sc-10671. Western blot analysis of BRIDGE-1 expression in 293T whole cell lysate.
