epitope mapping within an internal region of Med10 of human origin
recommended for detection of Med10 of mouse, rat and human origin by WB, IF and ELISA; also reactive with additional species, including canine, porcine and avian
Med10 Background Information The mediator complex is a multiprotein coactivator that is involved in the regulated transcription of Pol II-dependent genes. Functioning as a bridge to convey information from gene-specific regulatory proteins to the basal Pol II transcription machinery, the mediator complex is recruited to promoter regions by directly interacting with regulatory proteins. The mediator complex also serves as a scaffold for the assembly of a functional pre-initiation complex with Pol II and other general transcription factors. Med10 (Mediator of RNA polymerase II transcription subunit 10), also designated Transformation-related gene 17 protein, is a 135 amino acid nuclear protein that is a middle domain component of the mediator complex. Reduction of Med10 protein levels within cells leads to an enhancement of Wnt and Nodal signaling pathways. Also, in yeast, mutation the gene encoding Med10, as well as other middle domain mediator components, leads to enhanced transcription wild-type heat-shock genes.
