epitope mapping at the C-terminus of Med8 of human origin
recommended for detection of Med8 of mouse, rat and human origin by WB, IP, IF and ELISA; non cross-reactive with other Med family members; also reactive with additional species, including equine, canine, bovine, porcine and avian
Med8 Background Information In mammalian cells, transcription is regulated in part by high molecular weight co-activating complexes that mediate signals between transcriptional activators and RNA polymerase II (Pol II). The mediator complex is one such multi-protein structure that functions as a bridge between regulatory proteins and Pol II, thereby regulating Pol II-dependent transcription. Med8 (mediator complex subunit 8), also known as arc32 (activator-recruited cofactor 32 kDa component), is a 268 amino acid protein that localizes to the nucleus and exists as a component of the mediator complex. Involved in the pathway of protein modification and ubiquitination, Med8 is involved in transcriptional regulation and may also recruit E3 ubiquitin-protein ligase complexes to proteins targeted for proteasomal degradation. Multiple isoforms of Med8 exist due to alternative splicing events.
Med8 (C-13)
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Med8 (C-13): sc-103619. Western blot analysis of Med8 expression in Hep G2 whole cell lysate.
Med8 (C-13): sc-103619. Western blot analysis of Med8 expression in non-transfected: sc-117752 (A) and mouse Med8 transfected: sc-125596 (B) 293T whole cell lysates.
Med8 (C-13): sc-103619. Western blot analysis of Med8 expression in non-transfected: sc-117752 (A) and mouse Med8 transfected: sc-125596 (B) 293T whole cell lysates.
Med8 (C-13): sc-103619. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.
