epitope mapping within an internal region of EBF4 of human origin
recommended for detection of EBF4 of human origin by WB, IF and ELISA; non cross-reactive with family member EBF ; also reactive with additional species, including canine and porcine
EBF4 Background Information B lymphocyte maturation is an intricate process that requires a distinct set of transcription factors with respect to the stage of cell differentiation and cell lineage. Among the transcriptional regulators involved in the early stages of B cell development, EBF4 (early B-cell factor 4), also known as transcription factor COE4, is a 602 amino acid nuclear protein that binds the Olf1 site, the consensus sequence 5'-ATTCCCNNGGGAATT-3'. Like other members of the Olf-1/EBF (O/E) family of transcription factors, EBF4 may play an important role in B-cell maturation and neural development. There are two isoforms of EBF4 that exist as a result of alternative splicing events. In regards to transcriptional activation of a reporter construct, all EBF4 isoforms are weaker than EBF, EBF2 and EBF3. By interacting with other O/E family members, EBF4 most likely forms homodimers or heterodimers to regulate gene expression.
