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- mouse monoclonal IgG1; 200 µg/ml
- raised against retinoblastoma gene product /β-galactosidase fusion protein
- recommended for detection of Rb p110 of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
- agarose conjugate for IP studies, sc-102 AC, 500 µg/0.25 ml agarose
- HRP conjugate, sc-102 HRP, 200 µg/ml
- fluorescein (sc-102 FITC) and rhodamine (sc-102 TRITC) conjugates for immunofluorescence, 200 µg/ml
- phycoerythrin conjugate for intracellular FCM, sc-102 PE, 100 tests
- Alexa Fluor® 405 (sc-102 AF405), Alexa Fluor® 488 (sc-102 AF488) and Alexa Fluor® 647 (sc-102 AF647) conjugates for FCM or immunofluorescence; 100 µg/2 ml
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Ordering InformationProduct Citations
Recommended Support Products:
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| Species |
Gene Name |
Gene ID |
Chromosome Location |
Isoform (mRNA) Accession # |
Protein Accession # |
OMIM™ Number |
| Human |
RB1 |
5925 |
13q14.2 |
NM_000321 |
P06400
|
259500 |
| Mouse |
Rb1 |
19645 |
14 D3 |
|
P13405
|
N/A |
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Rb Background Information
Pediatric cancer retinoblastoma and the formation of other human tumors can be attributed to mutations in the retinoblastoma tumor suppressor gene. The retinoblastoma tumor suppressor gene product, known as Rb or pRb, regulates differentiation, apoptosis and cell cycle control by coordinating the cell cycle, at G1/S, with transcriptional machinery that includes the heterodimeric E2F family. During G1, cyclin D (D1, D2, D3)-dependent kinase-mediated phosphorylation of Rb at Ser 795 marks the conversion of Rb from a transcriptionally repressive, hypophosphorylated state to an inactive, phosphorylated state, which may be sustained through mitosis by differential phosphorylation of up to 16 putative serine or threonine residues, including Ser 249/Thr 252, Thr 373, Thr 356, Ser 780, Ser 807/Ser 811 and Thr 821/Thr 826. Hypophosphorylated Rb represses the transcription of genes controlling cell cycle through direct protein-protein interactions, by binding and inactivating the promoters of transcription factors, and through recruitment of histone deacetylase, which deacetylates promoter regions and enhances nucleosome formation, thereby masking transcription enhancing cis elements.
| Rb (IF8) Product Citations |
See how others have used Rb (IF8): sc-102 antibody and or Rb (IF8) antibody conjugates.
94 total citations Loading citations.
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Rb (IF8)
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Rb (IF8): sc-102. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue showing nuclear staining.
Western blot analysis of Rb p110 expression in A-431 whole cell lysate (A-C) and nuclear extract (D). Antibodies tested include Rb (C-15): sc-50 (A), Rb (C-15)-G: sc-50-G (B), Rb (IF8): sc-102 (C) and Rb (M-153): sc-7905 (D). Western blot analysis of Rb phosphorylation in SK-LMS-1 whole cell lysate. Blots were probed with p-Rb (Ser 807/811)-R: sc-16670-R preincubated with cognate phosphorylated peptide (A) or unphosphorylated peptide (B) and Rb (IF8): sc-102 (C). Gel supershift analysis of E2F proteins isolated by immunoprecipitation using either p107 (A,B) or Rb p110 (C-E) antibodies. E2F-4 released from p107/E2F complexes and incubated with 32P-labeled E2F binding sites specific oligonucleotide was supershifted by E2F-4 (C-108) X: sc-512 X antibody (B) as compared to control IgG (A). Similarly, E2F-4 released from Rb p110/E2F complexes was supershifted by E2F-4 (C-108) X: sc-512 X (E), but not by E2F-1 (KH95) X: sc-251 X (D) as compared to control IgG (C). Kindly provided by Masa-aki Ikeda, Ph.D., D.D.S. Rb siRNA (h): sc-29468. Western blot analysis of Rb expression in non-transfected control (A) and Rb siRNA transfected (B) HeLa cells. Blot probed with Rb (IF8): sc-102. γ Tubulin (C-11): sc-17787 used as specificity and loading control. Rb (IF8) Alexa Fluor 488: sc-102 AF488. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG 1: sc-3890. Rb (IF8) Alexa Fluor 647: sc-102 AF647. Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal mouse IgG 1: sc-24636. Rb (IF8): sc-102. Western blot analysis of Rb expression in non-transfected: sc-117752 (A) and human Rb transfected: sc-114014 (B) 293T whole cell lysates. Rb (IF8): sc-102. Western blot analysis of Rb expression in non-transfected 293T: sc-117752 (A), human Rb transfected 293T: sc-159907 (B) and K-562 (C) whole cell lysates. Rb (IF9): sc-102. Western blot analysis of Rb expression in K-562 whole cell lysate.
Rb (IF8) PE: sc-102 PE. Intracellular FCM analysis of fixed and permeabilized K-562 cells. Black line histogram represents the isotype control, normal mouse IgG 1: sc-2866. |
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