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p-MEK-1 Antibody (Thr 291): sc-101733

 |  Datasheet

(Based on data analysis)

  • p-MEK-1 Antibody (Thr 291) is a rabbit polyclonal IgG provided at 100 µg/ml
  • epitope corresponding to phosphorylated Thr 291 of MEK-1 of human origin
  • recommended for detection of Thr 291 phosphorylated MEK-1 of mouse, rat and human origin by WB and IP
 

See additional MEK Antibodies including MEK, MEK, MEK-1, MEK-2, MEK-3, MEK-4, MEK-5, MEK-6 and MEK-7.

Also see MEK-1 Inhibitors for functional analysis of cellular responses to MEK-1.


Ordering InformationGene Info
Recommended Support Products:
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WB   IP   siRNA  
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 Ordering Information
Product NameCatalog #UnitPriceQtyAdd 
p-MEK-1 Antibody (Thr 291) sc-101733 100 µg/ml $279
Lambda Phosphatase sc-200312 100000 units in 0.25 ml $462
Lambda Phosphatase sc-200312A 20000 units in 0.05 ml $116
Lambda Phosphatase sc-200312 100000 units in 0.25 ml $462
Lambda Phosphatase sc-200312A 20000 units in 0.05 ml $116
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
MEK-1 siRNA (h) sc-29396 10 µM $258
MEK-1 siRNA (m) sc-35904 10 µM $258
MEK-1 (h)-PR sc-29396-PR 10 µM, 20 µl $23
MEK-1 (m)-PR sc-35904-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
MEK-1 shRNA Plasmid (h) sc-29396-SH 20 µg $520
MEK-1 shRNA Plasmid (m) sc-35904-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
MEK-1 shRNA (h) Lentiviral Particles sc-29396-V 200 µl $625
MEK-1 shRNA (m) Lentiviral Particles sc-35904-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAdd 
T-47D Cell Lysate sc-2293 500 µg/200 µl $104
KNRK Whole Cell Lysate sc-2214 500 µg/200 µl $104
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human MAP2K1 5604 15q22.31 NM_002755 Q02750
 176872
Mouse Map2k1 26395 9 C P31938
 N/A
Mouse Map2k2 26396 10 C1 Q63932
 N/A
 

A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK (1). A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity (2). MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases (3). MEK-5 appears to specifically phosphorylate ERK5 (4), whereas MEK-6 phosphorylates p38 and p38ß (5,6). MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway (7–9). Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAFor MEKK1) positively regulates the kinase activity.
p-MEK-1 Antibody (Thr 291) Data
Click on image to enlarge
sc-101733
p-MEK-1 (Thr 291): sc-101733. Western blot analysis of phosphorylated MEK-1 expression in untreated (A) and serum-treated (B) 293 whole cell lysates.
sc-101733
p-MEK-1 (Thr 291): sc-101733. Western blot analysis of MEK-1 phosphorylation in EGF treated (A) and EGF and lambda protein phosphatase treated (B) 293T whole cell lysates.
sc-101733
Western blot analysis of MEK-1 phosphorylation in untreated (A, C) and lambda protein phosphatase treated (B, D) KNRK whole cell lysates. Antibodies tested include p-MEK-1 (Thr 291): sc-101733 (A, B) and MEK-1 (C-18): sc-219 (C, D).
sc-101733
Western blot analysis of MEK-1 phosphorylation in EGF treated (A, C) and EGF and lambda protein phosphatase treated (B, D) HEK293 whole cell lysates. Antibodies tested include p-MEK-1 (Thr 291): sc-101733 (A, B) and MEK-1 (C-18): sc-219 (C, D).
sc-101733
Western blot analysis of MEK-1 phosphorylation in untreated (A,D), NGF treated (B,E) and NGF and lambda protein phosphatase treated (C,F) PC-12 whole cell lysates. Antibodies tested include p-MEK-1 (Thr 291): sc-101733 (A,B,C) and MEK-1 (H-8): sc-6250 (D,E,F).
sc-101733
Western blot analysis of MEK-1 phosphorylation in untreated (A,D), serum-starved, PMA-treated (B,E), serum-starved, PMA and lambda protein phosphatase (sc-200312A) treated (C,F) HeLa whole cell lysates. Antibodies tested include p-MEK-1 (Thr 291): sc-101733 (A,B,C) and MEK-1 (H-8): sc-6250 (D,E,F).
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