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p-HSP 27 (Ser 15) Antibody: sc-101699 |
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- rabbit polyclonal IgG, 100 µg/ml
- epitope corresponding to phosphorylated Ser 15 of HSP 27 of human origin
- recommended for detection of Ser 15 phosphorylated HSP 27 of mouse, rat and human origin by WB, IP, IF and IHC(P)
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p-HSP 27 Background Information
HSP 27 is a constitutively expressed cytoplasmic protein that co-localizes to the nucleus upon stress-induced insult. Heat shock, cytokines and hormones are among the factors that stimulate the synthesis of HSP 27. The intracellular concentration of the mammalian heat shock protein, HSP 27, increases several-fold after heat shock and other metabolic stresses, and is closely associated with the acquisition of thermotolerance. MAP kinase-activated protein kinase-2 phosphorylates HSP 27 on Serine residues Ser 15, Ser 78 and Ser 82, which are phosphorylated in vivo in response to growth factors and heat shock. Ser 15, Ser 78 and Ser 82 occur in the sequence motif RXXS, which is recognized by ribosomal protein S6 kinase II.
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p-HSP 27 (Ser 15)
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p-HSP 27 (Ser 15): sc-101699. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.
p-HSP 27 (Ser 15): sc-101699. Western blot analysis of phosphorylated HSP 27 expression in untreated (A) and UV-treated (B) HeLa whole cell lysates.
p-HSP 27 (Ser 15): sc-101699. Western blot analysis of HSP 27 phosphorylation in untreated (A), heat shocked (B) and heat shocked and lambda protein phosphatase treated (C) HeLa whole cell lysates.
Western blot analysis of HSP 27 phosphorylation in untreated (A,D), UV irradiated (B,E) and UV irradiated and lambda protein phosphatase treated (C,F) HeLa whole cell lysates. Antibodies tested include p-HSP 27 (Ser 15): sc-101699 (A,B,C) and HSP 27 (F-4): sc-13132 (D,E,F). |
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