epitope corresponding to phosphorylated Thr 36 of 4E-BP1/2/3 of human origin
recommended for detection of Thr 36 phosphorylated 4E-BP1/2/3 of human origin and correspondingly phosphorylated Thr 35 of mouse and rat origin by WB, IP, IF and IHC(P)
p-4E-BP1/2/3 Background Information The multisubunit eukaryotic translation initiation factor (eIF) 4F recruits 40S ribosomal subunits to the 5' end of mRNA. The eIF4F subunit eIF4E interacts directly with the mRNA 5' cap structure. Assembly of the eIF4F complex is inhibited by a family of repressor polypeptides, the eIF4E-binding proteins (4E-BPs). 4E-BP1 (also known as PHAS-1) normally binds eIF4E, inhibiting cap-dependent translation. Hyper-phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. The PI3-kinase/Akt pathway and the FRAP/mTOR kinase regulate 4E-BP1. 4E-BP1 is phosphorylated in vivo on multiple residues and phosphorylation by FRAP/mTOR on Threonine 37 and Threonine 46 of human 4E-BP1 may prime it for sub-sequent phosphorylation at sites including Serine 65 and Threonine 70. The corresponding rat residues include Threonine 36, Threonine 45, Serine 64 and Threonine 69. In vitro, 4E-BP1 is also phosphorylated by ataxia telangiectasia (ATM) at human Serine 112 (rat Serine 111) in response to an increase in insulin levels.
p-4E-BP1/2/3 (Thr 36)
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p-4E-BP1/2/3 (Thr 36): sc-101624. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear and cytoplasmic localization.
p-4E-BP1/2/3 (Thr 36): sc-101624. Western blot analysis of phosphorylated 4E-BP1/2/3 expression in untreated (A) and EGF-treated (B) MDA-MB-435 whole cell lysates.
