RUNX3 Antibody (R3-5G4): sc-101553

RUNX3 Antibody (R3-5G4) is a mouse monoclonal IgG1 kappa light chain antibody that detects RUNX3 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. anti-RUNX3 antibody (R3-5G4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RUNX3, a member of the Runt-related transcription factor family, plays a crucial role in regulating gene expression related to cellular differentiation and cell cycle progression. RUNX3 is predominantly expressed in hematopoietic cells, including myeloid and B-cell lines, as well as in the spleen. RUNX3 is involved in the growth and differentiation of gastric epithelial cells, positioning RUNX3 as a significant candidate for gastric cancer tumor suppression. RUNX3 function is particularly important because hypermethylation of the RUNX3 gene can lead to inactivation, which has been implicated in the development of gastric cancer. Detection of hypermethylation within the RUNX3 CpG island can serve as a valuable biomarker for diagnosing and assessing the risk of gastric cancer, highlighting RUNX3′s importance in both cancer biology and potential therapeutic strategies.

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RUNX3 Antibody (R3-5G4) References:

1. Regulation of AML2/CBFA3 in hematopoietic cells through the retinoic acid receptor alpha-dependent signaling pathway.  |  Le, XF., et al. 1999. J Biol Chem. 274: 21651-8. PMID: 10419474
2. Oncogenic potential of the RUNX gene family: 'overview'.  |  Ito, Y. 2004. Oncogene. 23: 4198-208. PMID: 15156173
3. Epigenetic inactivation of the RUNX3 gene in lung cancer.  |  Sato, K., et al. 2006. Oncol Rep. 15: 129-35. PMID: 16328045
4. Spreading of methylation within RUNX3 CpG island in gastric cancer.  |  Homma, N., et al. 2006. Cancer Sci. 97: 51-6. PMID: 16367921
5. Role of Runx proteins in chondrogenesis.  |  Yoshida, CA. and Komori, T. 2005. Crit Rev Eukaryot Gene Expr. 15: 243-54. PMID: 16390320
6. Runx3 controls growth and differentiation of gastric epithelial cells in mammals.  |  Fukamachi, H. 2006. Dev Growth Differ. 48: 1-13. PMID: 16466388
7. Runx3 programs CD8+ T cell residency in non-lymphoid tissues and tumours.  |  Milner, JJ., et al. 2017. Nature. 552: 253-257. PMID: 29211713
8. Epigenetic reprogramming of Runx3 reinforces CD8 + T-cell function and improves the clinical response to immunotherapy.  |  Liu, Z., et al. 2023. Mol Cancer. 22: 84. PMID: 37189103
9. Cloning, mapping and expression of PEBP2 alpha C, a third gene encoding the mammalian Runt domain.  |  Bae, SC., et al. 1995. Gene. 159: 245-8. PMID: 7622058
10. AML-2 is a potential target for transcriptional regulation by the t(8;21) and t(12;21) fusion proteins in acute leukemia.  |  Meyers, S., et al. 1996. Oncogene. 13: 303-12. PMID: 8710369
11. A new transcription factor family associated with human leukemias.  |  Speck, NA. and Terryl, S. 1995. Crit Rev Eukaryot Gene Expr. 5: 337-64. PMID: 8834230
12. Rearrangements of the AML1/CBFA2 gene in myeloid leukemia with the 3;21 translocation: in vitro and in vivo studies.  |  Zent, C., et al. 1997. Leukemia. 11 Suppl 3: 273-8. PMID: 9209363

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-RUNX3 Antibody (R3-5G4) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-RUNX3 Antibody (R3-5G4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

RUNX3 Antibody (R3-5G4) Specifications

Alternate Product Name: RUNX3

Product Clone Name: R3-5G4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 191-300

Epitope Target: RUNX3

Epitope Species: human

Product Reactivity/Detection: RUNX3

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for RUNX3 Antibody (R3-5G4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin